G3907
グルタチオン-アガロ-ス
set of 3 pre-packed columns (2.5 ml each), (1:1 suspension in a 0.5 M NaCl + 20% ethanol solution)
別名:
GSH-アガロース結合, S-結合グルタチオンアガロース
製品名
グルタチオン-アガロ-ス, set of 3 pre-packed columns (2.5 ml each), (1:1 suspension in a 0.5 M NaCl + 20% ethanol solution)
form
(1:1 suspension in a 0.5 M NaCl + 20% ethanol solution)
analyte chemical class(es)
proteins (GST)
packaging
set of 3 pre-packed columns (2.5 ml each)
technique(s)
immunoprecipitation (IP): suitable
protein purification: suitable
matrix
cross-linked 4% beaded agarose
storage temp.
2-8°C
Quality Level
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関連するカテゴリー
Application
グルタチオンアガロ-スを用いたアフィニティ-クロマトグラフィ-では、グルタチオンS-トランスフェラ-ゼ(GST)、グルタチオンペルオキシダ-ゼ、グリオキサラ-ゼIなど、グルタチオン結合配列を含むタンパク質を、迅速かつ穏和で非変性性の条件の下、高い選択性で精製できます。
Affinity chromatography using glutathione-agarose permits rapid, mild, non-denaturing and highly selective purification of glutathione binding enzymes such as glutathione-S-transferase, glutathione peroxidase, and glyoxalase I.
The product was used in the design, synthesis and biological evaluation of new tryptamine and tetrahydro-β-carboline-based selective inhibitors of CDK4. It was also used to study Fascaplysin-inspired diindolyls as selective inhibitors of CDK4/cyclin D1.
The product was used in the design, synthesis and biological evaluation of new tryptamine and tetrahydro-β-carboline-based selective inhibitors of CDK4. It was also used to study Fascaplysin-inspired diindolyls as selective inhibitors of CDK4/cyclin D1.
Other Notes
製品番号G4510の懸濁液です。
General description
The resin in these columns consists of glutathione attached through the sulfur to epoxy activated, 4% cross-linked beaded agarose resulting in a 12 atom spacer.
Physical form
1:1 suspension in a 0.5 M NaCl + 20% ethanol solutionl
保管分類
10 - Combustible liquids
wgk
WGK 1
flash_point_f
180.1 °F
flash_point_c
82.3 °C
F Toribio et al.
Journal of chromatography. B, Biomedical applications, 684(1-2), 77-97 (1996-09-20)
The different preparative techniques and related analytical methods used for purification of glutathione peroxidase, glutathione transferase and glutathione reductase, described in papers published in the last ten years, have been reviewed in this article. Among the different purification techniques, chromatography
Paul R Jenkins et al.
Bioorganic & medicinal chemistry, 16(16), 7728-7739 (2008-07-25)
We present the design, synthesis and biological activity of a library of substituted (biphenylcarbonyl)-tryptamine and (biphenylcarbonyl)-tetrahydro-beta-carboline compounds related to the natural product fascaplysin, as novel inhibitors of CDK4/cyclin D1. We show all these molecules, prepared using the Suzuki-Miyaura reaction, being
Diane M Kanter et al.
Nucleic acids research, 39(7), 2580-2592 (2010-11-27)
Sld2 is essential for the initiation of DNA replication, but the mechanism underlying its role in replication is not fully understood. The S-phase cyclin dependent kinase (S-CDK) triggers the association of Sld2 with Dpb11, and a phosphomimetic mutation of Sld2
Purification of glutathione S-transferases from human liver by glutathione-affinity chromatography.
P C Simons et al.
Analytical biochemistry, 82(2), 334-341 (1977-10-01)
Xiu Feng Hu et al.
The Journal of clinical investigation, 119(2), 362-375 (2009-01-17)
Provirus integration site for Moloney murine leukemia virus (PIM1) is a proto-oncogene that encodes a serine/threonine kinase with multiple cellular functions. Overexpression of PIM-1 plays a critical role in progression of prostatic and hematopoietic malignancies. Here we describe the generation
関連コンテンツ
親和性、GSTプルダウン、TAP、共免疫沈降法を利用して、プルダウンアッセイでin vitroタンパク質間相互作用を調査します。
Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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