製品名
モノクローナル抗緑色蛍光タンパク質(GFP), N末端抗体 マウス宿主抗体, clone GSN24, purified from hybridoma cell culture
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
GSN24, monoclonal
form
buffered aqueous solution
mol wt
antigen 27 kDa
concentration
~2 mg/mL
technique(s)
western blot: 1-2 μg/mL using GFP fusion proteins expressed in extracts of transfected cells
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
2-8°C
target post-translational modification
unmodified
Quality Level
Biochem/physiol Actions
GFP融合タンパク質と特異的に反応します。
Green Fluorescent Protein (GFP) is a unique tool in cellular and molecular biology research to monitor gene expression and protein localization in living cells. In the jellyfish, A. victoria, GFP transduces the excitation energy resulting from emission of blue light of the photoprotein aequorin and reemits it as green light.
Physical form
0.01M PBS溶液(pH 7.4, 15mMアジ化ナトリウム含有)
Application
Anti-Green Fluorescent Protein (GFP), N-terminal antibody, Mouse monoclonal has been used in: western blotting, enzyme linked immunosorbent assay (ELISA), immunofluorescence labeling.
Disclaimer
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Anti-Green Fluorescent Protein (GFP), N-terminal antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma GSN24 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice.
Preparation Note
For continuous use, store at 2–8 °C for up to one month. For extended storage, store at –20 °C in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Solutions at working dilution should be discarded if not used within 12 hours.
適切な製品が見つかりませんか。
製品選択ツール.をお試しください
保管分類
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Marta Ukleja et al.
Nature communications, 7, 10433-10433 (2016-01-26)
CCR4-NOT is a large protein complex present both in cytoplasm and the nucleus of eukaryotic cells. Although it is involved in a variety of distinct processes related to expression of genetic information such as poly(A) tail shortening, transcription regulation, nuclear
Piet Kramer et al.
Frontiers in genetics, 7, 165-165 (2016-09-30)
The mitochondrial permeability transition pore plays a key role in programmed cell death and the induction of autophagy. Opening of the pore is regulated by the mitochondrial peptidyl prolyl-cis, trans-isomerase cyclophilin D (CYPD). Previously it was shown in the aging
Matthias Wiemer et al.
F1000Research, 3, 230-230 (2014-12-19)
The degradation of damaged proteins is an important vital function especially during aging and stress. The ubiquitin proteasome system is one of the major cellular machineries for protein degradation. Health and longevity are associated with high proteasome activity. To demonstrate
Mohammad I K Hamad et al.
Development (Cambridge, England), 141(8), 1737-1748 (2014-03-29)
The ionotropic α-amino-3-hydroxy-5-methyl-4-isoxazole propionate glutamate receptors (AMPARs) have been implicated in the establishment of dendritic architecture. The transmembrane AMPA receptor regulatory proteins (TARPs) regulate AMPAR function and trafficking into synaptic membranes. In the current study, we employ type I and
Design of inducible expression vectors for improved protein production in Ralstonia eutropha H16 derived host strains
Gruber S, et al.
Journal of Biotechnology, 235(8), 92-99 (2016)
関連コンテンツ
Instructions
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
製品に関するお問い合わせはこちら(テクニカルサービス)