H3274
ハイグロマイシンB Streptomyces hygroscopicus由来
powder, non-animal origin, suitable for cell culture and insect cell culture, BioReagent
別名:
Hygromycin
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この商品について
製品名
ハイグロマイシンB Streptomyces hygroscopicus由来, powder, BioReagent, suitable for cell culture, suitable for insect cell culture
biological source
Streptomyces hygroscopicus
Quality Level
product line
BioReagent
form
powder
concentration
≥60% (HPLC)
technique(s)
cell culture | insect: suitable, cell culture | mammalian: suitable
color
faintly brown to brown, white to beige
(1) 7.1, (2) 8.8
mp
160-180 °C
solubility
H2O: 50 mg/mL (As a stock solution. Stock solutions should be stored at 2-8°C. Stable at 37°C for 30 days.)
antibiotic activity spectrum
fungi
mode of action
protein synthesis | interferes
storage temp.
2-8°C
SMILES string
CN[C@H]1C[C@@H](N)[C@H](O)[C@@H](O[C@@H]2O[C@H](CO)[C@H](O)[C@@H]3O[C@]4(O[C@H]([C@H](N)CO)[C@H](O)[C@H](O)[C@H]4O)O[C@H]23)[C@@H]1O
InChI
1S/C20H37N3O13/c1-23-7-2-5(21)9(26)15(10(7)27)33-19-17-16(11(28)8(4-25)32-19)35-20(36-17)18(31)13(30)12(29)14(34-20)6(22)3-24/h5-19,23-31H,2-4,21-22H2,1H3/t5-,6-,7+,8-,9+,10-,11+,12-,13+,14-,15-,16+,17+,18-,19+,20+/m1/s1
InChI key
GRRNUXAQVGOGFE-XKIAHZFYSA-N
General description
Application
- プラスミド混合物を160 μg/mLでトランスフェクトされた293T細胞
- 150 μg/mLでのMCF-7ALOX12細胞。
- MSCVルシフェラーゼPGK-ヒグロプラスミド。
- 10% FBSを補充したDMEM中で維持されたSTAT3レポーターHeLa安定細胞株（STAT3プロモーターの制御下で安定的にルシフェラーゼ遺伝子をトランスフェクトされた）。
Biochem/physiol Actions
抗菌スペクトル：ヒグロマイシンBは、細菌、真菌およびより高等な真核細胞に対して作用します。
Preparation Note
Disclaimer
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signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 1 Inhalation - Acute Tox. 2 Dermal - Acute Tox. 2 Oral
保管分類
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
flash_point_f
Not applicable
flash_point_c
Not applicable
プロトコル
Stem Cell protocols for cryopreservation, thawing of cryopreserved stem cells and media preparation.
資料
Antibiotics targeting bacterial ribosomes disrupt protein synthesis, a key process in bacterial growth inhibition.
Antibiotic kill curve is a dose response experiment in which mammalian cells are subjected to increasing amounts of selection antibiotic
Prevent fungal, yeast, and mold contamination in cell cultures. Discover the best antifungal agent for your cultures with the extensive Sigma® antifungal collection.