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Merck

H9286

抗アセチル-ヒストンH3 (Ac-Lys9) ウサギ宿主抗体

2-2.5 mg/mL, affinity isolated antibody, buffered aqueous solution

別名:

Anti-H3K9ac

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この商品について

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD02685921
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ARR, IF, WB
Species reactivity:
bovine, human, Caenorhabditis elegans, frog, mouse, rat, Drosophila, chicken
Citations:
26
Technique(s):
indirect immunofluorescence: 1:1,000 using NIH3T3 mouse fibroblast cell line treated with sodium butyrate, microarray: suitable, western blot: 1:1,000 using whole cell extract of the mouse fibroblast NIH3T3 cell line treated with sodium butyrate
Uniprot accession no.:
P68431

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biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~17 kDa

species reactivity

bovine, human, Caenorhabditis elegans, frog, mouse, rat, Drosophila, chicken

concentration

2-2.5 mg/mL

technique(s)

indirect immunofluorescence: 1:1,000 using NIH3T3 mouse fibroblast cell line treated with sodium butyrate, microarray: suitable, western blot: 1:1,000 using whole cell extract of the mouse fibroblast NIH3T3 cell line treated with sodium butyrate

UniProt accession no.

P68431

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

acetylation (Lys9)

Quality Level

200

Gene Information

human ... H3F3A(3020), H3F3B(3021), HIST1H3A(8350), HIST1H3B(8358), HIST1H3C(8352), HIST1H3D(8351), HIST1H3E(8353), HIST1H3F(8968), HIST1H3G(8355), HIST1H3H(8357), HIST1H3I(8354), HIST1H3J(8356), HIST2H3A(333932), HIST2H3C(126961), HIST3H3(8290)
mouse ... H3f3a(15078), H3f3b(15081), Hist1h3a(360198), Hist1h3b(319150), Hist1h3c(319148), Hist1h3d(319149), Hist1h3e(319151), Hist1h3f(260423), Hist1h3g(97908), Hist1h3h(319152), Hist1h3i(319153), Hist2h3b(319154), Hist2h3c1(15077), Hist2h3c2(97114)
rat ... H3f3b(117056)

Immunogen

ヒトヒストンH3のN末端に相当する合成[Ac-Lys9]アセチル化ヒストンH3ペプチド(アミノ酸7-20)。この配列はマウス, ラット, ウシ, ニワトリ, カエル, Drosophila, C. elegansなど多くの生物種で同一であり、TetrahymenaヒストンH3でも高度に保存されている(1アミノ酸置換)。

Physical form

0.01M PBS溶液 (pH 7.4, 15mMアジ化ナトリウム含有)。

Application

Anti-acetyl-Histone H3 (Ac-Lys9) antibody produced in rabbit has been used in:
  • indirect immunofluorescence
  • immunoblotting
  • immunohistochemistry

Biochem/physiol Actions

Histone proteins H3, H4, H2A, and H2B function as building blocks to package eukaryotic DNA into repeating nucleosome units that are folded in higher order chromatin fibers.
In most species, histone H3 is primarily acetylated at lysine 9, 14, 18, and 23. Acetylation at lysine 9 significantly influences histone deposition and chromatin assembly in some organisms. Acetylation of specific lysine residues in H3 is also associated with processes apart from transcription.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Anti-acetyl-Histone H3 (Ac-Lys9) antibody produced in rabbit is suitable for:
  • indirect immunofluorescence at a dilution of 1:1,000 using NIH3T3 mouse fibroblast cell line treated with sodium butyrate
  • microarray
  • western blot at a dilution of 1:1,000 using whole cell extract of the mouse fibroblast NIH3T3 cell line treated with sodium butyrate

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保管分類

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

H9286-200UL: + IXO11355: + H9286-VAR: + H9286-BULK:

jan

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試験成績書（COA）

Lot/Batch Number
111M4857
089K4796
072K4824

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以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Genliang Li et al.
Cytotechnology, 69(1), 75-87 (2016-11-30)
As a well-known crustacean model species, the Chinese mitten crab Eriocheir sinensis presents spermatozoa with decondensed DNA. Our aim was to analyze structural distribution of the histone H3 and its acetylated lysine 9 (H3K9ac) during spermatogenesis for the mechanistic understanding
Gang Xi et al.
Molecular endocrinology (Baltimore, Md.), 22(9), 2162-2175 (2008-07-09)
Our previous studies have indicated an essential role of p52shc in mediating IGF-I activation of MAPK in smooth muscle cells (SMC). However, the role of the p66 isoform of shc in IGF-I signal transduction is unclear. In the current study
Social isolation-induced epigenetic changes in midbrain of adult mice
Siuda D, et al.
Journal of Physiology And Pharmacology, 65(2), 247-255 (2014)
Twenty-five years of the nucleosome, fundamental particle of the eukaryote chromosome.
R D Kornberg et al.
Cell, 98(3), 285-294 (1999-08-24)
Jamie Benoit et al.
Brain structure & function, 221(8), 3963-3978 (2015-11-04)
Histone acetylation is considered a major epigenetic process that affects brain development and synaptic plasticity, as well as learning and memory. The transcriptional effectors and morphological changes responsible for plasticity as a result of long-term modifications to histone acetylation are
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