M1567
リンゴ酸デヒドロゲナーゼ ブタ心臓由来
≥600 units/mg protein (biuret), ammonium sulfate suspension
別名:
L-リンゴ酸:NAD+オキシドレダクターゼ, MDH, リンゴ酸デヒドロゲナーゼ
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この商品について
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-622-5
MDL number:
製品名
リンゴ酸デヒドロゲナーゼ ブタ心臓由来, ≥600 units/mg protein (biuret), ammonium sulfate suspension
form
ammonium sulfate suspension
specific activity
≥600 units/mg protein (biuret)
foreign activity
Glutamic-Oxalacetic Transaminase ≤0.01%
Glutamic-Pyruvic Transaminase ≤0.01%
storage temp.
2-8°C
Quality Level
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General description
Malic dehydrogenase is a cytoplasmic isozyme and an important catalyst in the tricarboxylic acid cycle.
Malic Dehydrogenase is a ubiquitous enzyme, which exists in two isoforms in eukaryotic cells.
Malic dehydrogenase exists as a dimer with each subunit containing an NAD-binding domain and a substrate-binding carboxy-terminal domain required for activity.
Other Notes
1ユニットは、pH 7.5、25°C、1分間で、1.0 μmolのオキサロ酢酸とβ-NADHをL-リンゴ酸とβ-NADに変換する酵素量です。
Physical form
2.8 M (NH4)2SO4溶液(pH 6.0)の懸濁液
Application
Malic Dehydrogenase from porcine heart has been used:
- in qualitative protein binding measurements
- to test internally calibrated electrochemical continuous enzyme assay (ICECEA) with model enzyme pair
- to investigate the effect of chaperone on the refolding of heat-denatured malate dehydrogenase
Malic dehydrogenase has been used in a study to assess the effect of an immunomodulator S2 complex on the enzymes of the parasites. It has also been used in a study to investigate the heterogeneity of lactic and malic dehydrogenase in cerebrospinal fluid.
Biochem/physiol Actions
Malic Dehydrogenase (MDH) plays an important role in the citric acid cycle in mitochondria. It catalyzes the interconversion of substrates malate and oxaloacetate with the simultaneous oxidation/reduction of NAD/NADH+. MDH present in the cytosol is involved in the shuttling of malate/aspartate.
保管分類
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Yassir M Al-Mulla Hummadi et al.
Experimental parasitology, 112(2), 85-91 (2005-11-09)
S(2) complex has been reported to have a direct antileishmanial effect. The possibility that the direct antileishmanial effect may be due to inhibition of key enzymes involved in glucose metabolism and/ or enzymes associated with virulence was investigated. Cell pellets
Heterogeneity of lactic and malic dehydrogenase in cerebrospinal fluid
Lowenthal, A., et al.
Journal of Neurochemistry, 7, 135-140 (2006)
Electrochemical Coupled-Enzyme Assays at Carbon Nanotubes
Zhang M, et al.
Analytical Chemistry, 86(18), 9330-9334 (2014)
Malate dehydrogenases-structure and function
Minarik P, et al.
General Physiology and Biophysics, 21(3), 257-266 (2002)
Sensing NADH conformation using phasor analysis on fluorescence spectra
Palo D, et al.
Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy, 186(3), 105-111 (2017)
資料
硫酸アンモニウム懸濁液として提供される酵素の使用方法
Instructions for working with enzymes supplied as ammonium sulfate suspensions
プロトコル
Spectrophotometric assay evaluates malic dehydrogenase activity using bovine heart enzyme with critical histidine residue at active site.
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