M3769
199培地
Modified, with Earle′s salts, without L-glutamine, sodium bicarbonate, and phenol red, powder, suitable for cell culture
別名:
M199 Medium, TCM 199
製品名
199培地, Modified, with Earle′s salts, without L-glutamine, sodium bicarbonate, and phenol red, powder, suitable for cell culture
form
powder
technique(s)
cell culture | mammalian: suitable
components
L-glutamine: no
phenol red: no
HEPES: no
sodium pyruvate: no
Earle’s salts (5% CO2): yes
NaHCO3: no
shipped in
ambient
storage temp.
2-8°C
Quality Level
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関連するカテゴリー
General description
培地199は、初代移植片の培養のため完全既知組成培地として当初開発されたビタミン、アミノ酸、およびその他の因子の組み合わせです。この培地は、適切なサプリメントが添加される場合、特に形質転換していない細胞など、幅広い用途があります。培地199は、ワクチン生産、初代膵臓 外植片のin vitro培養、および水晶体組織に広く使用されます。
Preparation Note
0.1 g/LのL-グルタミンおよび2.2 g/Lの重炭酸ナトリウムを添加してください。
培地1 Lあたり粉末9.4 gが含まれる組成になっています。
Application
Medium 199 has been used:
- to culture the primary human umbilical vein endothelial cells (HUVECs) for use in cell viability assay
- to flush the uterine horn of gilts to recover day 12 conceptuses from uteri
- to culture the peritoneal cells and immortalized human mesothelial cells (MeT-5A and ATCC-CRL-9444)
保管分類
13 - Non Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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資料
Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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