M4642
イーグル最少必須培地
With Hanks′ salts and ʟ-glutamine, without sodium bicarbonate, powder, suitable for cell culture
別名:
MEM
製品名
イーグル最少必須培地, With Hanks′ salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
form
powder
technique(s)
cell culture | mammalian: suitable
components
glucose: yes
Hanks’ salts (2% CO2): yes
L-glutamine: yes
Earle’s salts (5% CO2): no
NaHCO3: no
phenol red: yes
sodium pyruvate: no
stable glutamine: no
HEPES: no
shipped in
ambient
storage temp.
2-8°C
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Preparation Note
0.35 g/L 炭酸水素ナトリウムを添加してください。
培地1 Lあたり粉末10.6 gが含まれる組成になっています。
Application
Minimum Essential Medium Eagle (MEM) has been used:
- as a component of the Hank′s MEM (HMEM)-Pipes buffer to induce anaphylatoxin induced-ear swelling in mice
- as a component of the slice culture medium (SCM) for the incubation of mouse hippocampal slice cultures
- to culture Müller cells from guinea pigs
General description
Minimum Essential Medium Eagle (MEM) is a synthetic cell culture media, developed by Harry Eagle. It has high concentrations of amino acids, so the medium closely approximates the protein concentration of cultured mammalian cells. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has increased the applicability of this medium. This medium lacks calcium that permits the growth of cells in suspension. Modification of the original MEM formulation. Earle′s salts have been replaced with Hanks′ salts. Since the bicarbonate concentration is substantially lower, this medium has a substantially reduced buffering capacity.
保管分類
11 - Combustible Solids
wgk
WGK 1
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
名称等を表示すべき危険物及び有害物
ishl_indicated
名称等を通知すべき危険物及び有害物
ishl_notified
M4642-2X5L: + M4642-25KG: + M4642-ALC: + M4642-50K: + M4642-50KG: + M4642-VAR: + M4642-25K: + M4642-10L: + M4642-BULK: + M4642LF1-BIBC: + M4642-BIBC: + M4642-1L: + M4642-PROC: + M4642-50L: + M4642-10X1L:
jan
The activation of IL-8 receptors in cultured guinea pig Muller glial cells is modified by signals from retinal pigment epithelium
Iwona M G, et al.
Journal of Neuroimmunology, 161, 49-60 (2005)
Anthony Dudilot et al.
Current biology : CB, 30(7), 1167-1176 (2020-02-23)
Synaptic plasticity, with its two most studied forms, long-term potentiation (LTP) and long-term depression (LTD), is the cellular mechanism underlying learning and memory. Although it has been known for two decades that bidirectional synaptic plasticity necessitates a corresponding bidirectional regulation
Simona Giunta et al.
The Journal of cell biology, 190(2), 197-207 (2010-07-28)
The signaling cascade initiated in response to DNA double-strand breaks (DSBs) has been extensively investigated in interphase cells. Here, we show that mitotic cells treated with DSB-inducing agents activate a "primary" DNA damage response (DDR) comprised of early signaling events
Pasqualino de Antonellis et al.
PloS one, 6(9), e24584-e24584 (2011-09-21)
Through negative regulation of gene expression, microRNAs (miRNAs) can function as oncosuppressors in cancers, and can themselves show altered expression in various tumor types. Here, we have investigated medulloblastoma tumors (MBs), which arise from an early impairment of developmental processes
Carlos Mendoza-Palomares et al.
Eukaryotic cell, 7(4), 684-697 (2008-02-19)
Cysteine proteases have been shown to be essential virulence factors and drug targets in trypanosomatids and an attractive antidisease vaccine candidate for Trypanosoma congolense. Here, we describe an important amplification of genes encoding cathepsin B-like proteases unique to T. congolense.
資料
A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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