M7292
M16培地
With sodium bicarbonate and lactic acid, without penicillin and streptomycin, liquid, sterile-filtered
別名:
M16 培地溶液, 細胞培養培地, 細胞培養培地 M16
製品名
M16培地, With sodium bicarbonate and lactic acid, without penicillin and streptomycin, liquid, sterile-filtered
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | embryo: suitable
impurities
endotoxin, tested
components
NaHCO3: 2.101 g/L
phenol red: 0.0106 g/L
sodium pyruvate: 0.0363 g/L
glucose: 1.0 g/L (Dextro)
shipped in
ambient
storage temp.
2-8°C
Quality Level
関連するカテゴリー
Analysis Note
本製品は、1細胞のマウス胚から拡張胚盤胞への発達を促進する能力が試験されています。最低要件として、80%が胚盤胞に発達するかどうかを確認しています。
溶液中に存在する粒子は製品の性能に影響しません。
Application
M2培地とM16培地は、着床前段階の胚のin vitro培養によく用いられる培地です。改変型クレブス・リンガー重炭酸塩溶液であり、ウィッテン培地と非常に似ています。着床前胚はグルコースを効率的に利用できないため、M16培地にはエネルギー源としてピルビン酸と乳酸が含まれています。
Preparation Note
0.06 g/L ペニシリンGカリウムおよび0.05 g/L 硫酸ストレプトマイシンを添加してください。
保管分類
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Qian-Qian Sha et al.
Nature communications, 9(1), 3477-3477 (2018-08-30)
Trimethylation of histone H3 on lysine-4 (H3K4me3) is associated with gene-regulatory elements, but its transcription-independent function in cell division is unclear. CxxC-finger protein-1 (CFP1) is a major mediator of H3K4 trimethylation in mouse oocytes. Here we report that oocyte-specific knockout
Xiao-Man Liu et al.
Oncotarget, 7(2), 1155-1167 (2015-12-31)
Mitochondrial dynamics change mitochondrial morphological features and numbers as a part of adaptive cellular metabolism, which is vital for most eukaryotic cells and organisms. A disease or even death of an animal can occur if these dynamics are disrupted. Using
Marie-Christine Birling et al.
Scientific reports, 7, 43331-43331 (2017-03-08)
Modelling Down syndrome (DS) in mouse has been crucial for the understanding of the disease and the evaluation of therapeutic targets. Nevertheless, the modelling so far has been limited to the mouse and, even in this model, generating duplication of
Marco Ragusa et al.
RNA biology, 16(9), 1237-1248 (2019-05-29)
Circular RNAs (circRNAs) have a critical role in the control of gene expression. Their function in spermatozoa (SPZ) is unknown to date. Twenty-eight genes, involved in SPZ/testicular and epididymal physiology, were given in circBase database to find which of them
Hong-Bin Liu et al.
Advanced science (Weinheim, Baden-Wurttemberg, Germany), 6(15), 1900295-1900295 (2019-08-14)
A number of genes involved in zygotic genome activation (ZGA) have been identified, but the RNA-binding maternal factors that are directly related to ZGA in mice remain unclear. The present study shows that maternal deletion of Igf 2bp2 (also commonly known
資料
Mouse embryo media and embryo validated reagents for transgenic mouse embryo culture
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
製品に関するお問い合わせはこちら（テクニカルサービス）