M7653
199培地
With Hanks′ salts and sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture
別名:
Cell Culture Medium, M199 Medium, Medium 199 Solution, TCM 199
製品名
199培地, With Hanks′ salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
L-glutamine: no
NaHCO3: yes
Hanks’ salts (2% CO2): yes
sodium pyruvate: no
phenol red: yes
HEPES: no
shipped in
ambient
storage temp.
2-8°C
Quality Level
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関連するカテゴリー
General description
オリジナルの培地199の改変。アール塩がハンクス塩で置換されています。ハンクス塩には、より低濃度の炭酸水素と、濃度と塩形態の異なる必須無機イオンが使用されています。
培地199は、初代移植片の培養のため完全既知組成培地として当初開発されたビタミン、アミノ酸、およびその他の因子の組み合わせです。この培地は、適切なサプリメントが添加される場合、特に形質転換していない細胞など、幅広い用途があります。培地199は、ワクチン生産、初代膵臓 外植片のin vitro培養、および水晶体組織に広く使用されます。
Preparation Note
0.1 g/LのL-グルタミンを添加してください。
保管分類
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Etienne de Coulon et al.
iScience, 24(2), 102041-102041 (2021-02-04)
A comprehensive understanding of mechano-electrical coupling requires continuous intracellular electrical recordings being performed on cells undergoing simultaneously in vivo like strain events. Here, we introduce a linear strain single-cell electrophysiology (LSSE) system that meets these requirements by delivering highly reproducible unidirectional
IGSF1 Does Not Regulate Spermatogenesis or Modify FSH Synthesis in Response to Inhibins or Activins.
Emilie Brûlé et al.
Journal of the Endocrine Society, 5(4), bvab023-bvab023 (2021-04-03)
Loss-of-function mutations in the X-linked immunoglobulin superfamily, member 1 (IGSF1) gene result in central hypothyroidism, often associated with macroorchidism. Testicular enlargement in these patients might be caused by increases in follicle-stimulating hormone (FSH) levels, as IGSF1 has been proposed to
Yining Li et al.
The Journal of biological chemistry, 292(6), 2301-2314 (2016-12-21)
Pituitary follicle-stimulating hormone (FSH) is an essential regulator of fertility in females and of quantitatively normal spermatogenesis in males. Pituitary-derived activins are thought to act as major stimulators of FSH synthesis by gonadotrope cells.
Benoît X E Desbiolles et al.
Microsystems & nanoengineering, 6, 67-67 (2021-09-28)
Volcano-shaped microelectrodes (nanovolcanoes) functionalized with nanopatterned self-assembled monolayers have recently been demonstrated to report cardiomyocyte action potentials after gaining spontaneous intracellular access. These nanovolcanoes exhibit recording characteristics similar to those of state-of-the-art micro-nanoelectrode arrays that use electroporation as an insertion
Matthias Imboden et al.
Nature communications, 10(1), 834-834 (2019-02-21)
Systematic investigations of the effects of mechano-electric coupling (MEC) on cellular cardiac electrophysiology lack experimental systems suitable to subject tissues to in-vivo like strain patterns while simultaneously reporting changes in electrical activation. Here, we describe a self-contained motor-less device (mechano-active
資料
Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.
関連コンテンツ
Discover the formulation for Medium 199 for cell culture applications, including vaccine development.
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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