製品名
モノクロナール抗MAPキナーゼ, 活性化/モノリン酸化 (リン酸化トレオニン ERK-1&2) マウス宿主抗体, ~2 mg/mL, clone ERK-PT115, purified immunoglobulin, buffered aqueous solution
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
ERK-PT115, monoclonal
form
buffered aqueous solution
mol wt
antigen, ERK-1 44 kDa
antigen, ERK-2 42 kDa
species reactivity
rat, human
concentration
~2 mg/mL
technique(s)
capture ELISA: suitable
immunocytochemistry: suitable
microarray: suitable
western blot: 0.5 μg/mL using extract of a rat fibroblast cell line, Rat1, activated with sorbitol
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... MAPK1(5594), MAPK3(5595)
rat ... Mapk1(116590), Mapk3(50689)
Biochem/physiol Actions
抗体は、スレオニン一リン酸化型および活性化された二リン酸化型のMAPキナ-ゼ(ERK-1, ERK-2)と特異的に反応します。非リン酸化型のMAPキナ-ゼ分子や二リン酸化型のJNKおよびp38 MAPKは認識されません。抗体が認識するエピト-プには、活性化MAPキナ-ゼの調節部位のリン酸化スレオニン残基(ERK-2の場合Thr183)が含まれています。
Immunogen
リン酸化型のERK活性化ル-プに相当する合成ペプチド(HTGFLpTEYVAT)。
Physical form
0.01M PBS溶液(pH 7.4, 15mMアジ化ナトリウム含有)。
Application
Monoclonal Anti-MAP Kinase, Activated/monophosphorylated (Phosphothreonine ERK-1&2) antibody produced in mouse has been used in:
- immunoblotting
- western blotting
- immunofluorescence
- enzyme linked immunosorbent assay (ELISA)
- immunochemistry
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Activated/ Monophosphorylated (Phosphothreonine ERK-1&2) (mouse IgG1 isotype) is derived from the ERK-PT115 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. Mitogen-activated protein kinase 1 (MAPK1) is mapped to human chromosome 22q11.22 and MAPK3 is present on human chromosome 16p11.2.
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保管分類
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Evaluation of the role of MAPK1 and CREB1 polymorphisms on treatment resistance, response and remission in mood disorder patients
Calati R, et al.
Progress in Neuro-Psychopharmacology & Biological Psychiatry, 44, 271-278 (2013)
Leader cell positioning drives wound-directed collective migration in TGFbeta-stimulated epithelial sheets
Chapnick DA and Liu X
Molecular Biology of the Cell, 25(10), 1586-1593 (2014)
Dual regulation of miRNA biogenesis generates target specificity in neurotrophin-induced protein synthesis
Huang YWA, et al.
Cell, 148(5), 933-946 (2012)
Transcriptional consequences of 16p11. 2 deletion and duplication in mouse cortex and multiplex autism families
Blumenthal I, et al.
American Journal of Human Genetics, 94(6), 870-883 (2014)
MAPKs: new JNK expands the group
Davis RJ
Trends in Biochemical Sciences, 19, 1027-1037 (1994)
関連コンテンツ
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