MAK145
Fluorometric Intracellular Ros Kit
sufficient for 200 fluorometric tests (red)
別名:
Intracellular ROS Fluorescence Assay
製品名
Fluorometric Intracellular Ros Kit, sufficient for 200 fluorometric tests (red)
usage
sufficient for 200 fluorometric tests (red)
detection method
fluorometric
relevant disease(s)
neurological disorders; cancer
storage temp.
−20°C
関連するカテゴリー
Application
Fluorometric intracellular reactive oxygen species (ROS) Kit has been used for detection of intracellular ROS in live cells.
This kit is suitable for the detection of intracellular ROS by fluorescence microplate reader or a fluorescent microscope.
Biochem/physiol Actions
The kit provides a sensitive, one-step fluorometric assay to detect intracellular ROS (especially superoxide and hydroxyl radicals) in live cells within 1 hour incubation. ROS react with a cell-permeable sensor, resulting in a fluorometric product (λex = 520/λem = 605 nm) proportional to the amount of ROS present The assay can be performed in either a 96 or 384 multiwell plate format using a fluorescence microplate reader.
General description
Reactive oxygen species (ROS) are generated as a result of the reduction of oxygen during aerobic respiration and by various enzymatic systems within the cell. At physiological levels, ROS contribute to cell signaling and host defense. Increased ROS generation, above the detoxification capacity of the biological system, results in oxidative stress and cellular damage. The main damage to cells results from the ROS-induced alteration of macromolecules such as polyunsaturated fatty acids in membrane lipids, essential proteins, and DNA. ROS has been implicated in disease states, such as Alzheimer′s disease, Parkinson′s disease, cancer, and aging.
保管分類
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Please refer to KIT Component information
pdsc
Please refer to KIT Component information
prtr
Please refer to KIT Component information
fsl
Please refer to KIT Component information
ishl_indicated
Please refer to KIT Component information
ishl_notified
Please refer to KIT Component information
cart
キットコンポーネントの情報を参照してください
jan
Knockdown of ferroportin accelerates erastin-induced ferroptosis in neuroblastoma cells.
Geng N, et al.
European Review for Medical and Pharmacological Sciences, 22(12), 3826-3836 (2018)
Oxidative metabolism drives inflammation-induced platinum resistance in human ovarian cancer.
Matassa D S, et al.
Cell Death and Differentiation, 23(9), 1542-1542 (2016)
Jianyong Yu et al.
Experimental and therapeutic medicine, 22(3), 946-946 (2021-07-27)
Salinomycin is a polyether antiprotozoal antibiotic that is widely used as an animal food additive. Some antifungal, antiparasitic, antiviral and anti-inflammatory activities have been reported for salinomycin. Recently, the anti-cancer effect of salinomycin has been demonstrated in breast cancer; however
Neurodegenerative diseases and oxidative stress.
Barnham K J, et al.
Nature Reviews. Drug Discovery, 3(3), 205-205 null
Yara E Elakkad et al.
Breast cancer (Dove Medical Press), 13, 675-689 (2021-12-23)
Female breast cancer is the most prevalent cancer worldwide. Emerging evidence shows that simvastatin (SIM) has promising anticancer activities. However, the underlying mechanisms are not fully elucidated. Increasing reports imply statins can modulate ferroptosis through disrupting reactive oxygen species (ROS)
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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