N5386
ミクロコッカスヌクレアーゼ Staphylococcus aureus由来
100-300 units/mg protein
別名:
MNase, エンドヌクレアーゼ, ミクロコッカス由来, ミクロコッカスエンドヌクレアーゼ
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この商品について
CAS番号:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-748-0
MDL number:
製品名
ミクロコッカスヌクレアーゼ Staphylococcus aureus由来, 100-300 units/mg protein
biological source
Staphylococcus aureus
form
powder
specific activity
100-300 units/mg protein
composition
Protein, ≥40% E1%/280 (, Balance primarily sodium acetate)
technique(s)
DNA extraction: suitable
DNA purification: suitable
suitability
suitable for molecular biology
application(s)
cell analysis
storage temp.
−20°C
Quality Level
Gene Information
Staphylococcus aureus subsp. aureus Mu50 ... nuc(1120790)
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Biochem/physiol Actions
DNAの5′-ホスホジエステル結合を加水分解します。
Other Notes
注:1 μmolユニット = 約85 A260ユニットです。ここで、1 A260ユニットは、反応液量が3.55 mLの場合に、pH 8.8、37°C、30分間に、ΔA260が1.0となる酵素量です。光路長 = 1 cm。
Application
Micrococcal Nuclease can be used for the digestion of chromatin to identify the location of nucleosomes.
Nuclease micrococcal from Staphylococcus aureus has been used in a study to monitor hybridization reactions with DNA. It has also been used in a study to investigate the optimal conditions for assay of staphylococcal nuclease.
保管分類
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Optimal conditions for assay of staphylococcal nuclease
KAMMAN, J. and S. TATINI
Journal of Food Science, 42, 421-424 (1977)
Ho-Ryun Chung et al.
PloS one, 5(12), e15754-e15754 (2011-01-06)
Eukaryotic genomes are packed into chromatin, whose basic repeating unit is the nucleosome. Nucleosome positioning is a widely researched area. A common experimental procedure to determine nucleosome positions involves the use of micrococcal nuclease (MNase). Here, we show that the
Use of micrococcal nuclease to monitor hybridization reactions with DNA.
D L Kacian et al.
Analytical biochemistry, 58(2), 534-540 (1974-04-01)
Ashish Kumar Singh et al.
Nature communications, 12(1), 7011-7011 (2021-12-03)
Numerous chromatin remodeling enzymes position nucleosomes in eukaryotic cells. Aside from these factors, transcription, DNA sequence, and statistical positioning of nucleosomes also shape the nucleosome landscape. The precise contributions of these processes remain unclear due to their functional redundancy in
Amnat Phetrungnapha et al.
Fish & shellfish immunology, 34(3), 875-884 (2013-01-22)
RNA interference (RNAi) plays a crucial role as an antiviral defense in several organisms including plants and invertebrates. An understanding of RNAi machineries especially protein components of the RNA-induced silencing complex (RISC) is essential for prior to applying RNAi as
関連コンテンツ
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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