P1034
イソチオシアン酸フェニル
Sigma Grade, 8.36 M, suitable for solid phase protein sequencing analysis, ≥99% (GC), liquid
別名:
PITC
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この商品について
化学式:
C6H5NCS
CAS番号:
分子量:
135.19
UNSPSC Code:
12352118
NACRES:
NA.31
PubChem Substance ID:
EC Number:
203-138-1
Beilstein/REAXYS Number:
471392
MDL number:
Assay:
≥99% (GC)
Form:
liquid
InChI key
QKFJKGMPGYROCL-UHFFFAOYSA-N
InChI
1S/C7H5NS/c9-6-8-7-4-2-1-3-5-7/h1-5H
SMILES string
S=C=Nc1ccccc1
grade
Sigma Grade
assay
≥99% (GC)
form
liquid
concentration
8.36 M
refractive index
n20/D 1.6515 (lit.)
bp
218 °C (lit.)
mp
−21 °C (lit.)
density
1.132 g/mL at 20 °C (lit.)
suitability
suitable for solid phase protein sequencing analysis
storage temp.
2-8°C
Quality Level
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関連するカテゴリー
Application
第1級及び第2級アミンの誘導体化試薬。エドマン分解によるペプチドシークエンシング及びHPCLによるアミノ酸分析に用いられます。
Phenyl isothiocyanate may be used in the polypeptide and protein sequencing at the subpicomole and subnanomole levels, respectively, by applying automated Edman chemistry followed by the analysis using high-performance liquid chromatography (HPLC) technique.
Packaging
1mLサイズ、アルゴン雰囲気下、フレームシール褐色アンプル入り。
10 mLサイズ、アルゴン雰囲気下、スクリューキャップ付褐色バイアル入り。
10 mLサイズ、アルゴン雰囲気下、スクリューキャップ付褐色バイアル入り。
General description
Phenyl isothiocyanate belongs to the class of aromatic compounds with isothiocyanate groups. It is typically used as a precolumn derivatization agent for the formation of phenylthiocarbamyl amino acids for their subsequent determination using chromatography techniques.
signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 3 Oral - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1
保管分類
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
wgk
WGK 3
flash_point_f
190.4 °F - closed cup
flash_point_c
88 °C - closed cup
ppe
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
第4類:引火性液体 + 第三石油類 + 危険等級III + 非水溶性液体
fsl
P1034-10ML: + P1034-BULK: + P1034-10X1ML: + P1034-VAR: + P1034-1ML: + P1034-1ML-KC:
jan
R F Ebert
Analytical biochemistry, 154(2), 431-435 (1986-05-01)
A method for analysis of phenylthiocarbamyl-amino acids by HPLC is described. Solvent A contains 0.05 M sodium acetate plus triethylamine (2.75 ml/liter) adjusted to pH 6.40 with phosphoric acid. Solvent B consists of 50% solvent A, 40% acetonitrile, and 10%
R L Heinrikson et al.
Analytical biochemistry, 136(1), 65-74 (1984-01-01)
Methods for the quantitative derivatization of amino acids with phenylisothiocyanate and for the separation and quantitation of the resulting phenylthiocarbamyl derivatives by reverse-phase high-performance liquid chromatography are described. Phenylthiocarbamylation of amino acids proceeds smoothly in 5 to 10 min at
Examination of automated polypeptide sequencing using standard phenyl isothiocyanate reagent and subpicomole high-performance liquid chromatographic analysis
Tempst P and Riviere L
Analytical Biochemistry, 183(2), 290-300 (1989)
Misako Aito-Inoue et al.
Journal of agricultural and food chemistry, 54(15), 5261-5266 (2006-07-20)
For the isolation and detection of food-derived peptides in blood, an approach based on the derivatization of peptides with phenyl isothiocyanate (PITC) was developed. This approach allows hydrophilic peptides to be resolved and specifically detected by reversed-phase (RP) HPLC. For
R Gheshlaghi et al.
Analytical biochemistry, 383(1), 93-102 (2008-08-30)
Modified resolution and overall separation factors used to quantify the separation of complex chromatography systems are described. These factors were proven to be applicable to the optimization of amino acid resolution in reverse-phase (RP) HPLC chromatograms. To optimize precolumn derivatization
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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