P2621
ホスホマンノースイソメラーゼ 大腸菌由来
recombinant, expressed in E. coli, ammonium sulfate suspension, ≥50 units/mg protein
別名:
D-マンノース-6-リン酸ケトールイソメラーゼ, PMI, マンノースリン酸イソメラーゼ
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この商品について
Specific activity:
≥50 units/mg protein
Recombinant:
expressed in E. coli
recombinant
expressed in E. coli
form
ammonium sulfate suspension
specific activity
≥50 units/mg protein
storage temp.
2-8°C
Quality Level
Other Notes
1 unitは、グルコースリン酸イソメラーゼとグルコース6リン酸デヒドロゲナーゼによる共役酵素系を用い、pH 7.6、25°C、1分間で、1.0 μmoleのD-マンノース6-リン酸をD-フルクトース6-リン酸に変換します。
Packaging
底の開いたガラス瓶内容物は内部に挿入され接着された円錐部に入っています。
Physical form
3.2 M硫酸アンモニウム中に分散させた懸濁液
Application
PMI is used to study cell wall synthesis and energy production. PMI has been used to study how EDTA and metal ions, such as Zn++, Co++, Fe++, Mn++ and Cu++., can affect recovery and thermal stability. It may be used to study PMI′s effect on various alginate biosynthetic enzymes such as phosphomannomutase (PMM), GDP-mannose pyrophosphorylase (GMP), and GDP-mannose dehydrogenase (GMD).
Biochem/physiol Actions
Phosphomannose Isomerase (PMI) catalyses the interconversion of mannose 6-phosphate (Man-6-P) and fructose 6-phosphate (Fru-6-P), which provides a link between glucose metabolism and mannosylation.
保管分類
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
P2621-2MG: + P2621-10MG: + P2621-BULK: + P2621-VAR:
jan
Thangamani Rajesh et al.
Applied microbiology and biotechnology, 93(4), 1685-1693 (2011-09-29)
Phosphomannose isomerases (PMIs) in bacteria and fungi catalyze the reversible conversion of D-fructose-6-phosphate to D-mannose-6-phosphate during biosynthesis of GDP-mannose, which is the main intermediate in the mannosylation of important cell wall components, glycoproteins, and certain glycolipids. In the present study
Daisuke Tsuji et al.
Annals of neurology, 69(4), 691-701 (2011-04-27)
Novel recombinant human lysosomal β-hexosaminidase A (HexA) was developed for enzyme replacement therapy (ERT) for Tay-Sachs and Sandhoff diseases, ie, autosomal recessive GM2 gangliosidoses, caused by HexA deficiency. A recombinant human HexA (Om4HexA) with a high mannose 6-phosphate (M6P)-type-N-glycan content
Matthew R Amos et al.
Applied and environmental microbiology, 77(3), 776-785 (2010-12-15)
Extracellular polysaccharide (EPS) is produced by diverse bacterial pathogens and fulfills assorted roles, including providing a structural matrix for biofilm formation and more specific functions in virulence, such as protection against immune defenses. We report here the first investigation of
Wenxia Fang et al.
Microbiology (Reading, England), 155(Pt 10), 3281-3293 (2009-07-04)
Phosphomannose isomerase (PMI) is an enzyme catalysing the interconversion of mannose 6-phosphate (Man-6-P) and fructose 6-phosphate (Fru-6-P). The reaction catalysed by PMI is the first committed step in the synthesis of mannose-containing sugar chains and provides a link between glucose
Susanne Brunner et al.
Plant biotechnology journal, 9(8), 897-910 (2011-03-29)
Plant resistance (R) genes are highly effective in protecting plants against diseases, but pathogens can overcome such genes relatively easily by adaptation. Consequently, in many cases R genes do not confer durable resistance in agricultural environments. One possible strategy to
資料
硫酸アンモニウム懸濁液として提供される酵素の使用方法
Instructions for working with enzymes supplied as ammonium sulfate suspensions
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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