P4069
アルカリホスファターゼ 大腸菌由来
buffered aqueous glycerol solution, 20-50 units/mg protein (in glycine buffer)
別名:
オルトリン酸-モノエステルホスホヒドロラーゼ(アルカリ性至適)
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この商品について
CAS番号:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-631-4
MDL number:
Specific activity:
20-50 units/mg protein (in glycine buffer)
Biological source:
Escherichia coli
Application
Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It may be used for protein labeling when high sensitivity is required. Product P4069 is provided as a buffered aqueous glycerol solution.
Phosphatase, Alkaline from Escherichia coli has been used:
- in fluorometric assay to determine its enzyme activity
- in the dephosphorylating enzyme cocktail for dephosphorylation of peptides
- in treating the nerve and HL60 cell lysate for β-hydroxy β-methylglutaryl-Coenzyme A (HMG-CoA) reductase activity
Biochem/physiol Actions
Alkaline phosphatase is a model enzyme for understanding phosphomonoesterase. It is used in various biochemical methods and enzyme linked immunosorbent assay (ELISA).
Alkaline phosphatase, from Escherichia coli, is a dimeric, non-glycosylated protein which mainly reside in the periplasmic space. Three known isoforms exist. The enzyme requires zinc, and is activated by magnesium. E. coli akaline phosphatase has a broad specificity for phosphate esters.
Physical form
5 mM Tris-HCl溶液(pH 7.4, 0.5 mM MgCl2, 0.5 mM ZnCl2, 50% (w/v) グリセロ-ル含有)
Analysis Note
タンパク量はビウレット法により決定しています。
Other Notes
1 unitは、pH 10.4、37°C、1分間で、1.0 μmoleのリン酸p-ニトロフェニルを加水分解します。
Disclaimer
pH 8で5 mMのEDTAに溶解したアルカリホスファターゼは、75°C、10分間で不可逆的に熱不活化されます。
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
保管分類
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
P4069-100UN-PW: + P4069-VAR: + P4069-BULK: + P4069-25UN: + P4069-25UN-PW: + P4069-100UN: + P4069-5UN:
jan
Phospho-iTRAQ data article: Assessing isobaric labels for the large-scale study of phosphopeptide stoichiometry
Glibert P, et al.
Data in Brief, 4(2), 60-65 (2015)
Sensitive fluorogenic substrate for alkaline phosphatase
Levine MN and Raines RT
Analytical Biochemistry, 418(2), 247-252 (2011)
The prion protein and cellular cholesterol homeostasis
Diomede L, et al.
Neurobiology of Lipids, 1(2), 8-14 (2002)
Lionel M Igaz et al.
The American journal of pathology, 173(1), 182-194 (2008-06-07)
TAR DNA-binding protein (TDP-43) has been recently described as a major pathological protein in both frontotemporal dementia with ubiquitin-positive inclusions (FTLD-U) and amyotrophic lateral sclerosis. However, little is known about the relative abundance and distribution of different pathological TDP-43 species
Jong Kil Lee et al.
The Journal of experimental medicine, 211(8), 1551-1570 (2014-07-23)
In Alzheimer's disease (AD), abnormal sphingolipid metabolism has been reported, although the pathogenic consequences of these changes have not been fully characterized. We show that acid sphingomyelinase (ASM) is increased in fibroblasts, brain, and/or plasma from patients with AD and
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