P5994
ホスファターゼの基質
40 mg tablets
別名:
4-ニトロフェニルリン酸 二ナトリウム塩 六水和物, pNPP 二ナトリウム塩 六水和物
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この商品について
化学式:
O2NC6H4OP(O)(ONa)2 · 6H2O
CAS番号:
分子量:
371.14
NACRES:
NA.54
PubChem Substance ID:
UNSPSC Code:
12352204
EC Number:
224-246-5
MDL number:
製品名
ホスファターゼの基質, 40 mg tablets
InChI key
KAKKHKRHCKCAGH-UHFFFAOYSA-L
InChI
1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2
SMILES string
O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1
form
tablet
concentration
36-44 mg/tablet
impurities
p-nitrophenol, essentially free
solubility
H2O: soluble, clear, colorless to yellow (1 tablet in 8 mL of water.)
storage temp.
−20°C
Quality Level
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関連するカテゴリー
Application
アルカリホスファタ-ゼおよび酸性ホスファタ-ゼによるELISA法で使用する呈色基質です。黄色い可溶性最終生成物を生じ、は405 nmで分光光度的に定量可能です。ｐNPP反応は、3N NaOHを用いて停止し、405 nmで吸光度を測定できます。
Phosphatase is a chromogenic substrate for use with alkaline and acid phosphatase ELISA procedures. It is been used for the colorimetric detection of CTB fusion proteins. Produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. The pNPP reaction may be stopped with 3 N NaOH and read at 405 nm.
Phosphatase substrate has been used as a substrate in alkaline phosphatase assay in serum samples, bone marrow stromal cells and human mesenchymal stem cells (hMSC).
The substrate has been used to determine the alkaline phosphatase (ALP) activity in human osteoblast-like Saos-2 cell lysates. ALP acts as an early marker of osteoblast differentiation that is expressed just before matrix mineralization begins.
Preparation Note
1錠あたり40 mgの基質を含有
Dissolve tablets to the desired concentration in either of the following buffers: 0.1 M glycine, pH 10.4, with 1 mM MgCl2 and 1 mM ZnCl2 or 1 M diethanolamine, pH 9.8, with 0.5 mM MgCl2. Typically, a pNPP concentration of 1 mg/mL is used.
General description
Human phosphatase-substrate networks are mapped based on the literature and protein-protein interaction (PPI) database. Based on gene expression data, the possible network of phosphatase-substrate-kinase is predicted.
Other Notes
Substrate for the determination of acid and alkaline phosphatases.
保管分類
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
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資料
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
関連コンテンツ
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