P6244
プロテインチロシンホスファターゼ 1B ヒト
recombinant, expressed in E. coli
別名:
PTP-1B
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この商品について
Specific activity:
≥20 units/mg protein
Recombinant:
expressed in E. coli
Concentration:
≥0.5 mg/mL
recombinant
expressed in E. coli
form
solution
specific activity
≥20 units/mg protein
mol wt
37.4 kDa
concentration
≥0.5 mg/mL
UniProt accession no.
relevant disease(s)
diabetes; obesity; cancer
shipped in
dry ice
storage temp.
−70°C
Quality Level
Gene Information
human ... PTPN1(5770)
General description
Protein tyrosine phosphatase 1B human (PTP1B) is encoded by the gene PTPN1. In human chromosome, the gene PTPN1 is localized on 20q13.13. The protein has 37 kDa catalytic domain and the C-terminal hydrophobic residues targets the protein to the cytoplasmic side of the endoplasmic reticulum membrane.
Application
Protein Tyrosine Phosphatase 1B (PTP1B) human has been used in treating cell lysate prior to immunoprecipitation and protein pull-down studies. It has also been used in PTP1B activity assay and also for the evaluation of inhibitors like betulinic acid (BA) and sodium orthovanadate (Na3VO4), and 6-chloro-3-formyl-7-methylchromone (CFM) on PTP1B.
Protein tyrosine phosphatase 1B (PTP1B) is a pharmaceutical target used to study the treatment of various diseases such as obesity, cancer and type II diabetes mellitus.
The inhibitory effect of 8-(4-chlorophenylthio)-cAMP and 8-(4-chlorophenylthio)-cGMP on protein tyrosine phosphatase-1B has been assayed.
Biochem/physiol Actions
PTP-1B is a recombinant, non-transmembrane protein expressed in E. coli containing amino acid residues 1-322 of human PTP-1B. It has a molecular mass of 37.4 kDa. It is an abundant intracellular enzyme that is thought to act as a negative regulator of certain signaling pathways. It dephosphorylates tyrosine-phosphorylated proteins and peptides, and is usually localized in the cytostolic domain of the ER.
PTP1B dephosphorylates c-Src in several human breast cancer cell lines has a regulatory role for PTP1B in the control of c-Src kinase activity. PTP1B is regulates the cardiovascular effects of leptin. PTP1B controls the JAK/STAT signaling pathway by dephosphorylation of JAK2.
Polymorphism in the gene PTPN1 is associated with type 2 diabetes and obesity.
Physical form
Supplied as a solution in 50 mM HEPES (pH 7.2), 1 mM EDTA, 5 mM DTT, and 0.05% NP-40
Other Notes
1 unitは、pH 7.2、30°C、1分間で、1.0 μmoleのリン酸化ペプチドEGFRフラグメント988-998を加水分解します。
保管分類
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
P6244-50UG: + P6244-50UG-PW: + P6244-VAR: + P6244-BULK:
jan
p66Shc couples mechanical signals to RhoA through FAK-dependent recruitment of p115-RhoGEF and GEF-H1
Wu RF, et al.
Molecular and Cellular Biology, MCB-00194 (2016)
A colorimetric biosensor based on guanidinium recognition for the assay of protein tyrosine phosphatase 1B and its inhibitors
Lv J, et al.
New. J. Chem., 41(23), 14414-14419 (2017)
Adsorption between TC-stabilized AuNPs and the phosphate group: application of the PTP1B activity assay
Lv J, et al.
Analyst, 140(23), 8017-8022 (2015)
Analysis of common PTPN1 gene variants in type 2 diabetes, obesity and associated phenotypes in the French population
Cheyssac C, et al.
BMC Medical Genetics, 7(1), 44-44 (2006)
p66Shc couples mechanical signals to RhoA through FAK-dependent recruitment of p115-RhoGEF and GEF-H1
Wu RF, et al.
Molecular and cellular biology, MCB-00194 (2016)
関連コンテンツ
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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