P8806
o-フェニレンジアミン 二塩酸塩
tablet, 3 mg substrate per tablet
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この商品について
化学式:
C6H4(NH2)2·2HCl
CAS番号:
分子量:
181.06
UNSPSC Code:
12352204
NACRES:
NA.83
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
3912045
General description
o-フェニレンジアミン二塩酸塩（OPD）は発色基質であり、西洋ワサビペルオキシダーゼ複合体を利用して、酵素結合免疫吸着アッセイ（ELISA）法で使用できます。この基質は、オレンジがかった茶色を呈し、分光分析で450 nmで読み取れる可溶性最終産物を生成します。OPD反応は、3N HClまたは3M H2SO4で停止させることができ、492 nmで読み取ります。
Preparation Note
1タブレットを0.05 Mリン酸-クエン酸バッファー(pH 5.0)で溶解し、必要な濃度に調整してください(通常OPDは0.4 mg/mLで使用されます)。使用直前に、基質バッファー溶液100 mLあたり新しい30%過酸化水素40 μLを加えてください。タブレットはホイルで個別包装されています。
signalword
Warning
hcodes
Hazard Classifications
Aquatic Chronic 2 - Carc. 2 - Muta. 2 - Skin Sens. 1
保管分類
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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資料
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
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