Quality Level
form
powder
solubility
water: 10 mg/mL, clear to very slightly hazy (faint yellow to light yellow)
suitability
suitable for (suitable for use as a substrate for Rnase)
storage temp.
−20°C
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関連するカテゴリー
Application
パン酵母（S. cerevisiae）由来リボ核酸（RNA）は、ノーザンブロットハイブリダイゼーション、逆転写ポリメラーゼ連鎖反応（RTPCR）、およびcDNA作製などの用途に適しています。’
General description
パン酵母（S. cerevisiae）由来のリボ核酸（RNA）は、RNase酵素の基質です。’ 酵母からのRNAの単離は複雑なプロセスで、フェニルや界面活性剤（SDS）の存在下で細胞を何回も加熱したり凍結したりします。
Preparation Note
Crestfield, A.M., et al., J. Biol. Chem., 216, 185 (1955)の変法にて調製しています。
保管分類
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Lukas Bossaller et al.
Journal of immunology (Baltimore, Md. : 1950), 197(4), 1044-1053 (2016-06-30)
Systemic lupus erythematosus (SLE) is a chronic, life-threatening autoimmune disorder, leading to multiple organ pathologies and kidney destruction. Analyses of numerous murine models of spontaneous SLE have revealed a critical role for endosomal TLRs in the production of autoantibodies and
Hidekazu Iioka et al.
Nucleic acids research, 39(8), e53-e53 (2011-02-09)
The diverse localization of transcripts in cells suggests that there are many specific RNA-protein interactions that have yet to be identified. Progress has been limited, however, by the lack of a robust method to detect and isolate the RNA-binding proteins.
Michael R Green et al.
Cold Spring Harbor protocols, 2021(12) (2021-12-03)
Isolation of RNA from yeast is complicated by the need to first break the thick, rigid cell wall. The protocol provided here uses a cycle of heating and freezing of cells in the presence of phenol and the detergent sodium
Stephanie C Bannister et al.
Biology of reproduction, 81(1), 165-176 (2009-04-10)
MicroRNAs are a highly conserved class of small RNAs that function in a sequence-specific manner to posttranscriptionally regulate gene expression. Tissue-specific miRNA expression studies have discovered numerous functions for miRNAs in various aspects of embryogenesis, but a role for miRNAs
Hilal Kazan et al.
Nucleic acids research, 41(Web Server issue), W180-W186 (2013-06-12)
RBPmotif web server (http://www.rnamotif.org) implements tools to identify binding preferences of RNA-binding proteins (RBPs). Given a set of sequences that are known to be bound and unbound by the RBP of interest, RBPmotif provides two types of analysis: (i) de
プロトコル
This procedure may be used for determination of Ribonuclease A (RNase A) activity.
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