製品名
RPMI-1640培地, Modified, with sodium bicarbonate, without methionine, cystine and L-glutamine, liquid, sterile-filtered, suitable for cell culture
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
HEPES: no
sodium pyruvate: no
phenol red: yes
L-glutamine: no
NaHCO3: yes
shipped in
ambient
storage temp.
2-8°C
Quality Level
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General description
RPMI 1640 培地は、1966 年に、ムーアらによって、ロズウェルパーク記念研究所において開発されました。マッコイ5A 培地は、懸濁培養におけるリンパ芽球様細胞の維持のために設計されましたが、その後はさまざまな種類の足場依存性細胞にも使用できることが示されています。′当初は血清補助剤とともに使用することを意図していたRPMI1640は、血清非存在下でいくつかの細胞株を支持することが示されています。融合プロトコルおよびハイブリッド細胞の増殖でも広く使用されています。この培地は、ヒトの正常白血球と腫瘍性白血球の培養に適しています。
保管分類
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Md Abdullah et al.
Biomolecules & therapeutics (2021-10-21)
Targeting the cystine/glutamate exchange transporter, system xc -, is a promising anticancer strategy that induces ferroptosis, which is a distinct form of cell death mediated by iron-dependent lipid peroxidation. The concentration of L-cystine in culture medium is higher than the
Nathanael G Lintner et al.
PLoS biology, 15(3), e2001882-e2001882 (2017-03-23)
Proprotein convertase subtilisin/kexin type 9 (PCSK9) plays a key role in regulating the levels of plasma low-density lipoprotein cholesterol (LDL-C). Here, we demonstrate that the compound PF-06446846 inhibits translation of PCSK9 by inducing the ribosome to stall around codon 34
Polyamines Control eIF5A Hypusination, TFEB Translation, and Autophagy to Reverse B Cell Senescence.
Hanlin Zhang et al.
Molecular cell, 76(1), 110-125 (2019-09-03)
Failure to make adaptive immune responses is a hallmark of aging. Reduced B cell function leads to poor vaccination efficacy and a high prevalence of infections in the elderly. Here we show that reduced autophagy is a central molecular mechanism
Michael D Lewis et al.
The American journal of tropical medicine and hygiene, 81(6), 1041-1049 (2009-12-10)
Trypanosoma cruzi, the agent of Chagas disease, can be subdivided into six discrete typing units (DTUs), TcI, TcIIa, TcIIb, TcIIc, TcIId or TcIIe, each having distinct epidemiologically important features. Dozens of genetic markers are available to determine the DTU to
Sven D Willger et al.
PLoS pathogens, 4(11), e1000200-e1000200 (2008-11-08)
At the site of microbial infections, the significant influx of immune effector cells and the necrosis of tissue by the invading pathogen generate hypoxic microenvironments in which both the pathogen and host cells must survive. Currently, whether hypoxia adaptation is
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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