R7638
RPMI-1640培地
Dutch Modification, with sodium bicarbonate and HEPES, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture
別名:
Roswell Park Memorial Institute 1640 medium
製品名
RPMI-1640培地, Dutch Modification, with sodium bicarbonate and 20mM HEPES, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
pH
>7.2
components
sodium pyruvate: no
HEPES: 20 mM
L-glutamine: no
NaHCO3: yes
phenol red: yes
shipped in
ambient
storage temp.
2-8°C
Quality Level
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関連するカテゴリー
General description
RPMI 1640 培地は、1966 年に、ムーアらによって、ロズウェルパーク記念研究所において開発されました。マッコイ5A 培地は、懸濁培養におけるリンパ芽球様細胞の維持のために設計されましたが、その後はさまざまな種類の足場依存性細胞にも使用できることが示されています。′当初は血清補助剤とともに使用することを意図していたRPMI1640は、血清非存在下でいくつかの細胞株を支持することが示されています。融合プロトコルおよびハイブリッド細胞の増殖でも広く使用されています。この培地は、ヒトの正常白血球と腫瘍性白血球の培養に適しています。
Preparation Note
0.3 g/LのL-グルタミンを添加してください。
Application
RPMI-1640 Medium has been used
- to obtain spleen and lymph nodes from mice
- for the resuspension of Staphylococcus aureus strains in the phagocytosis assay
- in the isolation of hepatitis B surface antigen-specific B-cell clones
保管分類
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Kamila R Santos et al.
Vaccines, 9(8) (2021-08-29)
Staphylococcus aureus mastitis remains a major challenge for dairy farming. Here, 24 mice were immunized and divided into four groups: G1: control; G2: Granulocyte Macrophage Colony-Stimulating Factor (GM-CSF) DNA vaccine; G3: F0F1 ATP synthase subunit α (SAS), succinyl-diaminopimelate (SDD), and
Antonella Cerino et al.
PloS one, 10(4), e0125704-e0125704 (2015-04-30)
We describe the production and characterization of human monoclonal antibodies (mAb) specific for the major hepatitis B virus (HBV) S protein. The mAbs, two IgG1κ and one IgG1λ, were secreted by B-cell clones obtained from peripheral blood mononuclear cells (PBMC)
Michael D Lewis et al.
The American journal of tropical medicine and hygiene, 81(6), 1041-1049 (2009-12-10)
Trypanosoma cruzi, the agent of Chagas disease, can be subdivided into six discrete typing units (DTUs), TcI, TcIIa, TcIIb, TcIIc, TcIId or TcIIe, each having distinct epidemiologically important features. Dozens of genetic markers are available to determine the DTU to
Sven D Willger et al.
PLoS pathogens, 4(11), e1000200-e1000200 (2008-11-08)
At the site of microbial infections, the significant influx of immune effector cells and the necrosis of tissue by the invading pathogen generate hypoxic microenvironments in which both the pathogen and host cells must survive. Currently, whether hypoxia adaptation is
Ryoichi Arita et al.
Diabetes, 58(1), 215-226 (2008-10-09)
Leukocyte adhesion in retinal microvasuculature substantially contributes to diabetic retinopathy. Involvement of the Rho/Rho kinase (ROCK) pathway in diabetic microvasculopathy and therapeutic potential of fasudil, a selective ROCK inhibitor, are investigated. Localization of RhoA/ROCK and Rho activity were examined in
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