RAB0500

Human p53 ELISA Kit

Name: Human p53 ELISA Kit
Brand: SIGMA

for serum, plasma, cell culture supernatant and urine

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この商品について

NACRES:

NA.32

UNSPSC Code:

41116158

Gene information:

human ... TP53(7157)

Input:

sample type cell culture supernatant(s)
sample type urine
sample type serum
sample type plasma

Species reactivity:

human

Storage temp.:

−20°C

Shipped in:

wet ice

主要文書

すべての文書を表示

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species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable, capture ELISA: suitable

input

sample type cell culture supernatant(s)
sample type urine
sample type serum
sample type plasma

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 0.3 ng/mL
standard curve range: 0.328-80 ng/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... TP53(7157)

General description

Cellular tumor antigen p53 is a tumor-suppressor protein that comprises transactivation at N-terminus, central sequence-specific DNA-binding domain, and regulatory domain at C-terminus. It plays a key role in autophagy regulation. It inhibits tumors and acts as a cellular stress sensor especially during nutrient deprivation, hypoxia, and ribosome dysfunction. The Human p-53 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human p-53 in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human p53

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Human p53 ELISA Kit has been used for quantifying p53 using enzyme-linked immunosorbent assay (ELISA) in human myeloma RPMI-8226 cells, human colorectal cancer HCT-116 cells, human breast cancer MCF-7 cells and human leukemiaHL-60 cells.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

キットの構成要素は別途購入可能です。

製品番号

詳細

SDS

RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS

RABELADDELISA 5X Assay/Sample Diluent Buffer D (Item K)SDS

RABSTOP3ELISA Stop Solution (Item I)SDS

RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS

RABWASH420X Wash Buffer (Item B)SDS

pictograms

GHS05

signalword

Warning

hcodes

H290

pcodes

P234 - P390

Hazard Classifications

Met. Corr. 1

保管分類

10 - Combustible liquids

適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Please refer to KIT Component information

pdsc

Please refer to KIT Component information

prtr

Please refer to KIT Component information

fsl

Please refer to KIT Component information

ishl_indicated

Please refer to KIT Component information

ishl_notified

Please refer to KIT Component information

cart

キットコンポーネントの情報を参照してください

jan

最新バージョンのいずれかを選択してください：

試験成績書（COA）

Lot/Batch Number

適切なバージョンが見つかりませんか。

特定のバージョンが必要な場合は、ロット番号またはバッチ番号で特定の証明書を検索できます。

COAを検索

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Anticancer activity of newly synthesized 1,1-disubstituted cyclohexane-1-carboxamides: in vitro caspases mediated apoptosis activators in human cancer cell lines and their molecular modeling.

Walaa Hamada Abd-Allah et al.

Drug development research, 80(7), 933-947 (2019-07-26)

Novel 1,1-disubstituted cyclohexane-1-carboxamides 6a-h, 7a-e, and 8a-b were designed and synthesized as apoptotic inducers. Cytotoxicity test revealed that some compounds have strong to moderate effect, while others displayed weak action against different cancer cell lines including, MCF-7, HepG2, A549, and

Evaluation of roscovitine anticancer agent as a reference compound for cancer and apoptosis studies

Taha H, et al.

JIMD Reports null

Design, Synthesis and Screening of 4,6-Diaryl Pyridine and Pyrimidine Derivatives as Potential Cytotoxic Molecules.

Mohammed K Abd Elhameid et al.

Chemical & pharmaceutical bulletin, 66(10), 939-952 (2018-08-17)

A new series of pyridine and pyrimidine derivatives is designed and synthesized as potential antitumor molecules. The tested compounds show promising in vitro cytotoxic activity against HL-60 cell line as eight compounds: 4, 6, 11, 13, 14, 15, 18 and

p53 at a glance.

Colleen A Brady et al.

Journal of cell science, 123(Pt 15), 2527-2532 (2010-10-14)

Design, synthesis, and cytotoxicity screening of 5-aryl-3-(2-(pyrrolyl) thiophenyl)-1, 2, 4-oxadiazoles as potential antitumor molecules on breast cancer MCF-7 cells.

Mohammed K Abd El Hameid et al.

Bioorganic chemistry, 86, 609-623 (2019-02-27)

The work representing the design and the cytotoxic screening of synthetic small molecules (SSMs) such as carbonitriles 3a-c, carboximidamides 4a-c, and oxadiazoles 5-19 as antitumor molecules. Molecules 4c, 9, 12, and 14 show promising cytotoxicity profiles against two cell lines

Human p53 ELISA Kit

for serum, plasma, cell culture supernatant and urine

サイズを選択してください

この商品について

主要文書

特性

species reactivity

packaging

technique(s)

input

assay range

detection method

shipped in

storage temp.

Gene Information

関連するカテゴリー

詳細

General description

Immunogen

Application

Other Notes

キットの構成要素は別途購入可能です。

安全性情報

pictograms

signalword

hcodes

pcodes

Hazard Classifications

保管分類

適用法令

Please refer to KIT Component information

Please refer to KIT Component information

Please refer to KIT Component information

Please refer to KIT Component information

Please refer to KIT Component information

Please refer to KIT Component information

キットコンポーネントの情報を参照してください

資料

試験成績書（COA）

以前この製品を購入いただいたことがある場合

参考文献

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