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Merck

RK20407

ABScript II One Step RT-qPCR Probe Kit

別名:

One Step RT-qPCR master mix, Probe-based RT-qPCR

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usage

sufficient for 100 reactions, sufficient for 20 reactions, sufficient for 500 reactions

feature

dNTPs included, hotstart

technique(s)

DNA amplification: suitable, PCR: suitable, RT-PCR: suitable, RT-qPCR: suitable, cDNA synthesis: suitable, qPCR: suitable

input

purified RNA

compatibility

ABI 7000, ABI 7300, ABI 7500, ABI 7700, ABI 7900, ABI StepOne, ABI StepOnePlus, ABI ViiA 7, Bio-Rad CFX384, Bio-Rad CFX96, Bio-Rad iCycler, Eppendorf® Mastercycler ep realplex2 s, Eppendorf® Mastercycler ep realplex, Qiagen Corbett Rotor-Gene 3000, Qiagen Corbett Rotor-Gene 6000, Qiagen Corbett Rotor-Gene Q, QuantStudio™, Roche LightCycler 480, Strategene Mx3000P, Strategene Mx3005P, Strategene Mx4000

shipped in

dry ice

storage temp.

−20°C

General description

The ABScript II One Step RT-qPCR Probe Kit is a convenient, ready-to-use kit with optimized components for use for reverse transcription and subsequent probe-based qPCR. The one-step format prevents contamination and allows for higher sensitivity. This kit is ideal for analyzing low input RNA samples.The ABScript II Reverse Transcriptase in the kit provides reliable reverse transcription of RNA. After reverse transcription, the hot-start version of Taq polymerase is activated at 95°C and the ABScript II Reverse Transcriptase is inactivated simultaneously. In the sequential PCR reaction, the 5′-3′ exonuclease activity of Taq polymerase cleaves the hybridized probe, separating the reporter from the quencher and releasing fluorescent signal.

Application

ABScript II One Step RT-qPCR Probe Kit is designed for use in real-time RT-PCR (or RT-qPCR) applications, including detection of RNA in samples being analyzed.

For Research Use Only.

Application Note

Additional Materials Required but Not Included:

  • RNA templates, primers and probes
  • Optical-grade qPCR tubes, plates, sealing films, and aerosol-resistant pipette tips

Compatible Instruments:

*50X ROX Reference Dye I
Applied Biosystems 7000/7300/7700/7900, Applied Biosystems StepOne™/StepOnePlus™

**50X ROX Reference Dye II
Applied Biosystems 7500/ViiA™7, QuantStudio, Stratagene Real-time PCR Systems, Rotor-gene® 3000

NO ROX Reference Dye
Bio-Rad iCyclers/ CFX96™/ CFX384™, Roche® Light Cyclers, QIAGEN/Corbett Systems, Eppendorf Mastercycler®.

Features and Benefits

  • Ready-to-use one-step RT-qPCR probe kit with all components for RT-qPCR (except primers, probes and template RNA)
  • Uses the robust ABScript II Reverse Transcriptase (RT), which provides reliable and efficient reverse transcription on a wide range of RNA template amount
  • This one-step kit improves sensitivity and effectively prevents contamination
  • Flexible format, as the ABScript II One Step RT-qPCR Probe Kit includes two separate 50X concentrations of the ROX (passive reference) dye, to be added as required by specific qPCR instruments.

Other Notes

2X One Step RT-qPCR Probe Buffer*
One Step Probe HS Taq
ABScript II RT Enzyme Mix
50X ROX Dye I *
50X ROX Dye II **
RNase-free ddH2O
See datasheet for more information.

Reaction Size
Reaction size is based on a 50 uL reaction volume.

The 2x One Step RT-qPCR Probe Buffer is supplied in the following volumes:

20RXN: 0.5 mL

100RXN: 2.5 mL

500RXN: 1.25 mL

Legal Information

CFX384 is a trademark of Bio-Rad Laboratories, Inc.
CFX96 is a trademark of Bio-Rad Laboratories, Inc.
Eppendorf is a registered trademark of Eppendorf AG
Mastercycler is a registered trademark of Eppendorf AG
QuantStudio is a trademark of IROA Technologies LLC
Roche is a registered trademark of Roche Molecular Systems, Inc.
Rotor-Gene is a registered trademark of Qiagen GmbH
StepOne is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
StepOnePlus is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
ViiA is a trademark of ThermoFisher

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

保管分類

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

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prtr

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fsl

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ishl_indicated

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ishl_notified

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関連コンテンツ

RT-qPCR – 定量的逆転写PCR

RT-qPCRは、遺伝子発現や病原体検出における用途で、特定のターゲットを検出します。

Data Sheet - RK20407
RT-qPCR – Quantitative Reverse Transcription PCR

RT-qPCR detects specific targets with applications in gene expression and pathogen detection.

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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