製品名
炭酸水素ナトリウム 溶液, solution (7.5%), sterile-filtered, BioReagent, suitable for cell culture
sterility
sterile-filtered
product line
BioReagent
form
solution (7.5%)
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
pH
7.8-8.2
storage temp.
2-8°C
SMILES string
[Na+].OC([O-])=O
InChI
1S/CH2O3.Na/c2-1(3)4;/h(H2,2,3,4);/q;+1/p-1
InChI key
UIIMBOGNXHQVGW-UHFFFAOYSA-M
General description
重炭酸ナトリウム（NaHCo3）は、実験室で一般に用いられるアルカリ性の緩衝剤(5)です。このバッファーを細胞培養培地に添加すれば、インキュベータ内に存在する二酸化炭素を利用することによる適切なpHの維持に役立ちます。緩衝特性に加えて、重炭酸ナトリウムはナトリウムの原料としての役割も担い、また発酵過程ではグルコン酸ナトリウム合成を助けます。
Application
重炭酸ナトリウム溶液は、次の用途で使用されています：
- ハンクス平衡塩溶液（HBSS）の成分およびラットまたはマウス由来組織外植片の培養のための記録用培地の成分として
- ′マウス組織外植片の培養のためのダルベッコ改変イーグル培地（DMEM）の成分として
- ヒト結腸直腸腺がん細胞と乳腺腺がんに対する細胞毒性を調べるための最小必須培地（MEM）の成分として
- 3Dコラーゲン発芽アッセイ用のコラーゲンミックス液滴を調製するため
- ラット表皮角化細胞（REK）器官型培養用のコラーゲン基質を調製するため
Preparation Note
細胞培養グレ-ド水で調製されています。
保管分類
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Nguyen T K Vo et al.
Developmental and comparative immunology, 92, 140-149 (2018-11-20)
Viral double-stranded (ds)RNA is a potent pathogen-associated molecular pattern (PAMP), capable of inducing a strong antiviral state within the cell, protecting the cell from virus infection. In mammals and fish, sensing extracellular dsRNA is mediated by cell-surface class A scavenger
Mette Flinck et al.
Cell cycle (Georgetown, Tex.), 17(9), 1056-1067 (2018-06-14)
Precise acid-base homeostasis is essential for maintaining normal cell proliferation and growth. Conversely, dysregulated acid-base homeostasis, with increased acid extrusion and marked extracellular acidification, is an enabling feature of solid tumors, yet the mechanisms through which intra- and extracellular pH
Anna E Engler et al.
Cell reports, 33(13), 108553-108553 (2020-12-31)
There is an increasing appreciation for the heterogeneity of myeloid lineages in the lung, but relatively little is known about populations specifically associated with the conducting airways. We use single-cell RNA sequencing, flow cytometry, and immunofluorescence to characterize myeloid cells
Wenqing Zhou et al.
Journal of cell science, 133(17) (2020-08-14)
Neutrophils rely on glycolysis for energy production. How mitochondria regulate neutrophil function is not fully understood. Here, we report that mitochondrial outer membrane protein Mitofusin 2 (MFN2) regulates neutrophil homeostasis and chemotaxis in vivo Mfn2-deficient neutrophils are released from the
Le P Ngo et al.
Nucleic acids research, 48(3), e13-e13 (2019-12-12)
Genotoxicity testing is critical for predicting adverse effects of pharmaceutical, industrial, and environmental chemicals. The alkaline comet assay is an established method for detecting DNA strand breaks, however, the assay does not detect potentially carcinogenic bulky adducts that can arise
プロトコル
3-D Culture Matrix™ Collagen I facilitates cellular growth and differentiation in vitro, offering versatile applications in biomedical research.
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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