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この商品について
NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
5C5, monoclonal
Species reactivity:
rat, chicken, bovine, mouse, guinea pig, rabbit, human, planaria(flatworm), fish, Xenopus
Application:
ELISA, IF, IHC
Citations:
20
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
5C5, monoclonal
form
buffered aqueous solution
mol wt
antigen ~42 kDa
species reactivity
rat, chicken, bovine, mouse, guinea pig, rabbit, human, planaria(flatworm), fish, Xenopus
packaging
antibody small pack of 25 μL
technique(s)
ELISA: suitable, immunoblotting: 1:500-1:1000 using rat heart tissue extracts, immunofluorescence: 1:500-1000 using human HeLa cells., immunohistochemistry: 1:500 using formalin-fixed, paraffin-embedded human tongue sections and Biotin/ExtrAvidin®-Peroxidase staining system.
isotype
IgM
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... ACTA2(59)
General description
Monoclonal Anti-Actin (-Sarcomeric) (mouse IgM isotype) is derived from the hybridoma 5C5 produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with purified rabbit striated muscle. Actin is the major cytoskeletal protein in eukaryotic cells.
Immunogen
purified rabbit striated muscle
Application
Anti-Actin (α-Sarcomeric) antibody, Mouse monoclonal has been used in:
- indirect immunofluorescence staining
- immunohistochemistry
- enzyme-linked immunosorbent assay (ELISA)
- immunoblotting
- immunofluorescence
Biochem/physiol Actions
Actin plays essential roles in a number of cellular processes including cell migration, cytokinesis, vesicle transport and contractile force generation.
Physical form
The product is supplied as a culture supernatant solution containing 15 mM sodium azide as a preservative. The product contains bovine serum albumin and a human-derived protein.
Legal Information
ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
SAB4200689-BULK: + SAB4200689-VAR: + SAB4200689-25UL: + SAB4200689-100UL:
jan
Actin depolymerisation and crosslinking join forces with myosin II to contract actin coats on fused secretory vesicles
Miklavc P, et al.
Journal of Cell Science, 128(6), 1193-1203 (2015)
Clarkson, E., et al.,
The Journal of Pathology, 204, 407-417 (2004)
Babai, F., et al.,
Virchows Archiv. B, Cell Pathology Including Molecular Pathology, 55, 263-277 (1988)