Cycloheximide

A convenient ready-to-use form of Cycloheximide (Cat. No. 239763). An anti-fungal antibiotic that inhibits protein synthesis in eukaryotes but not in prokaryotes. Interacts directly with the translocase enzyme, interfering with the translocation step. Also inhibits cell-free protein synthesis in eukaryotes. Competitively inhibits hFKBP12 (K_i = 3.4 µM). Triggers apoptosis in HL-60 cells, T-cell hybridomas, Burkitt′s lymphoma cells, and a variety of other cell types, including rodent macrophages. Induces DNA fragmentation in macrophages, but inhibits DNA cleavage in rat thymocytes treated with thapsigargin, methylprednisolone, and ionomycin. Rapidly destroyed in alkaline solutions.

Primary Target
hFKBP12

Packaged under inert gas

A 100 mg/ml solution of Cycloheximide (Cat. No. 239763 ) in DMSO (sterile-filtered).

Following initial use, aliquot and refrigerate (4°C).

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Obrig, T.G., et al. 1971. J. Biol. Chem.246, 174.
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Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Toxicity: Highly Toxic & Carcinogenic / Teratogenic (I)