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324716

O-Glycosidase

Name: O-Glycosidase
Brand: MM

Hydrolyzes O-linked glycans, suitable for mass spectrometry, recombinant, expressed in E. coli

Synonym(s):

Endo-α-N-acetylgalactosaminidase, Streptococcus pneumoniae, Recombinant, E. coli, O-Glycopeptide endo-D-galactosyl-N-acetyl-α-galactosaminohydrolase, O-Glycosidase

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About This Item

CAS Number:

59793-96-3

EC Number:

3.2.1.97 (BRENDA, IUBMB)

MDL number:

MFCD00007030

NACRES:

NA.42

UNSPSC Code:

12352202

Specific activity:

≥1 units/mL, ≥10 units/mg protein

Recombinant:

expressed in E. coli

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Properties

Product Name

Endo-α-N-acetylgalactosaminidase, Streptococcus pneumoniae, Recombinant, E. coli, Endo-α-N-acetylgalactosaminidase, Streptococcus pneumoniae, Recombinant, E. coli, CAS 59793-96-3, catalyzes the hydrolysis of the unsubstituted Galβ1,3GalNAc core disaccharide attached to Ser or Thr.

recombinant

expressed in E. coli

Quality Segment

100

conjugate

(O-linked)

form

liquid

specific activity

≥1 units/mL, ≥10 units/mg protein

manufacturer/tradename

Calbiochem^®

storage condition

do not freeze

foreign activity

N-acetylglucosaminidase, α- and β-galactosidase, α-mannosidase, neuraminidase, proteases, none detected

shipped in

wet ice

storage temp.

2-8°C

Description

General description

Note: 1 mU = 1 milliunit.

Recombinant, Streptococcus pneumoniae Endo-α-N-acetylgalactosaminidase expressed in E. coli. Catalyzes the hydrolysis of the unsubstituted Galβ1,3GalNAc core disaccharide attached to serine or threonine residues of glycopeptides and glycoproteins to afford free oligosaccharides. For carbohydrates containing sialic acid or fucose, pretreatment with neuraminidase or fucosidase is required.

Physical form

In 50 mM sodium phosphate buffer, pH 7.5.

Other Notes

One unit is defined as the amount of enzyme that will catalyze the release of 1.0 µmol p-nitrophenol from p-nitrophenyl-2-acetamido-2-deoxy-3-O-(β-D-galactopyranosyl)-α-D-galactopyranoside per min at 37°C, pH 5.0.

Wang, A.M., et al. 1998. Mol. Genet. Metab. 65, 165.
Iwase, H., and Hotta, K. 1993. Methods Mol. Biol. 14, 151.
Fan, J.Q., et al. 1990. Agric. Biol. Chem. 54, 233.
Umemoto, J., et al. 1978. Anal. Biochem. 91, 186.
Glasgow, L.R., et al. 1977. J. Biol. Chem. 252, 8615.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Storage Class

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c