Anti-Protein Gene Product 9.5 Antibody

Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UniProt: P09936; also known as UCH-L1, Neuron cytoplasmic protein 9.5, PGP 9.5 (PGP9.5), Ubiquitin thioesterase L1) is encoded by the UCHL1 gene (Gene ID: 7345) in human. Protein gene product 9.5 (PGP9.5) is the historical name for ubiquitin C-terminal hydrolase L1 (UCHL1), a cysteine protease of the UCH deubiquitinase family that is highly enriched in neurons and widely used as a pan-neuronal/neuroendocrine marker in histopathology. Structurally, PGP9.5 adopts the canonical UCH fold and contains a catalytic Cys–His–Asp triad (human Cys90/His161/Asp176), with crystallographic analyses showing conformational features of the active site and ligand-induced rearrangements upon binding ubiquitin-based inhibitors/substrates that help rationalize its substrate selectivity and catalytic tuning. PGP9.5 participates in ubiquitin homeostasis by hydrolyzing C-terminal adducts from ubiquitin and processing ubiquitinated proteins, thereby interfacing with the broader ubiquitin–proteasome system (UPS) that governs proteostasis in long-lived cells such as neurons. PGP9.5 protein is strongly detected across neuronal cell types, predominantly localized to the cytosol with a substantial fraction reported as membrane-associated in brain, consistent with roles at neurites/synapse-proximal compartments and dynamic protein quality control. PGP9.5 has been discussed in the context of Parkinson’s disease genetics (e.g., the rare familial I93M variant and the debated S18Y polymorphism) and in neurodegeneration models implicating altered PGP9.5 enzymatic activity and downstream metabolic/proteostatic consequences.

KLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal of human Protein Gene Product 9.5.

Research Category
Neuroscience

Research Sub Category
Neuronal & Glial Markers

Anti-Protein Gene Product 9.5 Guinea Pig polyclonal antibody that detects Protein Gene Product 9.5. It targets an epitope within 17 amino acids from the C-terminal.

Evaluated by Immunohistochemistry (Paraffin) Analysis in human cerebellum tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution of this antibody detected UCHL1 in human cerebellum tissue sections.

Tested Applications

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected UCHL1 in human pancreas, mouse brain and rat brain tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target molecular weight 24.82 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Recognizes Protein Gene Product 9.5 (PGP9.5).

Unpurified

Guinea Pig polyclonal antibody in serum with 0.05% sodium azide.

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Control
Brain tissue

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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