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341088

1,9-Dimethyl-Methylene Blue zinc chloride double salt

Dye content 80 %

Synonym(s):

DMMB, Taylor’s Blue

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About This Item

Empirical Formula (Hill Notation):
C18H22ClN3S · 0.5ZnCl2
CAS Number:
931418-92-7
Molecular Weight:
416.05
NACRES:
NA.47
PubChem Substance ID:
24860900
UNSPSC Code:
12171500
MDL number:
MFCD19686959
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form

powder

Quality Segment

200

composition

Dye content, 80%

technique(s)

titration: suitable

impurities

<20% water

mp

250 °C (dec.) (lit.)

λmax

649 nm

ε (extinction coefficient)

≥10000 at 243-249 nm in H2O, ≥18000 at 285-291 nm in H2O, ≥25000 at 594-600 nm in H2O, ≥35000 at 646-652 nm in H2O

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].[Cl-].Cl[Zn]Cl.CN(C)c1cc(C)c2N=C3C(C)=C\C(C=C3Sc2c1)=[N+](/C)C.CN(C)c4cc(C)c5N=C6C(C)=C\C(C=C6Sc5c4)=[N+](/C)C

InChI

1S/2C18H22N3S.4ClH.Zn/c2*1-11-7-13(20(3)4)9-15-17(11)19-18-12(2)8-14(21(5)6)10-16(18)22-15;;;;;/h2*7-10H,1-6H3;4*1H;/q2*+1;;;;;+2/p-4

InChI key

MKLBAKXCEQNMBC-UHFFFAOYSA-J

Application

DMMB is a cationic dye that specifically binds to sulfated glycosaminoglycans (sGAG) with an absorbance at ~525 nm.
DMMB is a cationic dye that specifically binds to sulfated glycosaminoglycans (sGAG) with an absorbance at ~525 nm. It has been used to quantitate glycosaminoglyans such as chondroitin sulfate, keratin sulfate, and heparan sulfate and sGAG chains in proteoglycans. One limitation to this method The sGAG-DMMB complex is unstable in solution and may precipitate. Precipitation of the complex was shown to improve stability.
1,9-Dimethyl-Methylene Blue zinc chloride double salt has been used for the quantification of sulfated glycosaminoglycans.

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H319

Hazard Classifications

Eye Irrit. 2

Storage Class

11 - Combustible Solids

wgk

WGK 3

ppe

dust mask type N95 (US), Eyeshields, Gloves

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