50227
D-Luciferin potassium salt
≥98.0% (HPLC)
Synonym(s):
(S)-2-(6-Hydroxy-2-benzothiazolyl)-2-thiazoline-4-carboxylic acid potassium salt, 4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid potassium salt, Firefly luciferin potassium salt
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About This Item
Empirical Formula (Hill Notation):
C11H7KN2O3S2
CAS Number:
Molecular Weight:
318.41
UNSPSC Code:
12352106
NACRES:
NA.32
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
5416262
Quality Level
assay
≥98.0% (HPLC)
form
powder or crystals
solubility
water: soluble
storage temp.
−20°C
SMILES string
[K+].Oc1ccc2nc(sc2c1)C3=N[C@H](CS3)C([O-])=O
InChI
1S/C11H8N2O3S2.K/c14-5-1-2-6-8(3-5)18-10(12-6)9-13-7(4-17-9)11(15)16;/h1-3,7,14H,4H2,(H,15,16);/q;+1/p-1/t7-;/m1./s1
InChI key
UMBKGTQQGYPQBE-OGFXRTJISA-M
Application
D-Luciferin may be used as a substrate in luciferase-based (EC 1.13.12.7) bioluminescence imaging and cell-based high-throughput screening applications. ATP can be measured with a reagent made up of D-luciferin and luciferase from firefly.D-Luciferin may be used and studied as a partial agonist for G protein-coupled receptor-35 (GPR35).
Packaging
Bottomless glass bottle. Contents are inside inserted fused cone.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Yuanyuan Qiu et al.
ACS nano, 12(8), 7974-7985 (2018-08-07)
The inability to intraoperatively diagnose and eliminate microscopic residual tumors represents a significant challenge in cancer surgery. These residual microtumors cause lethal recurrence and metastasis. Herein, we show a crucial example of Raman imaging with gap-enhanced Raman tags (GERTs) to
Ellen Siebring-van Olst et al.
Journal of biomolecular screening, 18(4), 453-461 (2012-11-01)
The firefly luciferase gene is commonly used in cell-based reporter assays. Convenient luciferase assay reagents for use in high-throughput screening (HTS) are commercially available. However, the high cost of these reagents is not within the means of some academic laboratories.
Yimao Zhang et al.
Molecular imaging, 11(6), 499-506 (2012-10-23)
Bioluminescence imaging (BLI) detects light generated by luciferase-mediated oxidation of substrate and is used widely for evaluating transgene expression in cell-based assays and in vivo in relevant preclinical models. The most commonly used luciferase for in vivo applications is firefly