94046
Sodium phosphate dibasic solution
BioUltra, 0.5 M in H2O
Synonym(s):
Disodium hydrogen phosphate solution, di-Sodium hydrogen phosphate solution
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About This Item
product line
BioUltra
Quality Level
form
liquid
concentration
0.5 M in H2O
impurities
insoluble matter, passes filter test, ≤0.001% total nitrogen (N)
pH
8.8-9.4 (25 °C, 0.5 M in H2O)
solubility
H2O: 0.5 M at 20 °C, clear, colorless
density
1.064 g/mL at 20 °C
anion traces
chloride (Cl-): ≤10 mg/kg, sulfate (SO42-): ≤50 mg/kg
cation traces
Al: ≤1 mg/kg, As: ≤0.1 mg/kg, Ba: ≤1 mg/kg, Bi: ≤1 mg/kg, Ca: ≤5 mg/kg, Cd: ≤1 mg/kg, Co: ≤1 mg/kg, Cr: ≤1 mg/kg, Cu: ≤1 mg/kg, Fe: ≤1 mg/kg, K: ≤20 mg/kg, Li: ≤1 mg/kg, Mg: ≤1 mg/kg, Mn: ≤1 mg/kg, Mo: ≤1 mg/kg, Ni: ≤1 mg/kg, Sr: ≤1 mg/kg, Zn: ≤1 mg/kg
λ
0.5 M in H2O
UV absorption
λ: 260 nm Amax: 0.019, λ: 280 nm Amax: 0.016
application(s)
diagnostic assay manufacturing
SMILES string
[Na+].[Na+].OP([O-])([O-])=O
InChI
1S/2Na.H3O4P/c;;1-5(2,3)4/h;;(H3,1,2,3,4)/q2*+1;/p-2
InChI key
BNIILDVGGAEEIG-UHFFFAOYSA-L
Application
- to supplement Dulbecco′s Modified Eagle Medium (DMEM) in calcifying medium to induce the calcification of vascular smooth muscle cells (VSMCs)
- to prepare a stock solution of CaCl2 and MgCl2 to synthesize calcium phosphate (CPB) and magnesium phosphate bions (MPBs) respectively
- to supplement 2xYT agar plates to culture BL21 DE3 star cells and BL21dLac Gold cells
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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Protocols
To measure alcohol dehydrogenase activity, this assay uses β-nicotinamide adenine dinucleotide phosphate and a continuous spectrophotometric rate determination at 340 nm.
To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.