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Merck

B4261

Biocytin

≥98% (TLC), suitable for drug transporter assays

Synonym(s):

Bct, Nε-(+)-Biotinyl-L-lysine

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About This Item

Empirical Formula (Hill Notation):
C16H28N4O4S
CAS Number:
Molecular Weight:
372.48
NACRES:
NA.26
PubChem Substance ID:
UNSPSC Code:
12352209
MDL number:
Beilstein/REAXYS Number:
97197
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Product Name

Biocytin, ≥98% (TLC)

Quality Level

assay

≥98% (TLC)

form

powder

optical activity

[α]20/D +53°, c = 1.05% in 0.1 M NaOH(lit.)

technique(s)

drug transporter assay: suitable

color

white to off-white

mp

~245 °C (dec.) (lit.)

storage temp.

−20°C

SMILES string

[H][C@]12CS[C@@H](CCCCC(=O)NCCCC[C@H](N)C(O)=O)[C@@]1([H])NC(=O)N2

InChI

1S/C16H28N4O4S/c17-10(15(22)23)5-3-4-8-18-13(21)7-2-1-6-12-14-11(9-25-12)19-16(24)20-14/h10-12,14H,1-9,17H2,(H,18,21)(H,22,23)(H2,19,20,24)/t10-,11-,12-,14-/m0/s1

InChI key

BAQMYDQNMFBZNA-MNXVOIDGSA-N

Application

Biocytin is used as a substrate to study the specificity and kinetics of biotinidase(s), to measure biotinidase deficiency and as a model compound to study various uptake mechanisms of cells and tissues. Biocytin has been used to label neurons, medium spiny neurons and optic nerves.

Biochem/physiol Actions

Biocytin is an adduct of lysine and biotin. It is hydrolyzed by biotinidase, which acts on peptide-incorporated biotin or free biotin. Biocytin acts as a coenzyme.


Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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