C3306
Calcium chloride dihydrate
Molecular Biology, ≥99.0%
Synonym(s):
CaCl2.2H2O, Calcium chloride, Calcium dichloride
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About This Item
Empirical Formula (Hill Notation):
CaCl2 · 2H2O
CAS Number:
Molecular Weight:
147.01
NACRES:
NA.31
PubChem Substance ID:
UNSPSC Code:
12352302
EC Number:
233-140-8
MDL number:
Assay:
≥99.0%
Grade:
Molecular Biology
Form:
powder
Solubility:
H2O: 294 mg/mL, clear, colorless
grade
Molecular Biology
Quality Level
assay
≥99.0%
form
powder
mp
176 °C (dec.) (lit.)
solubility
H2O: 294 mg/mL, clear, colorless
foreign activity
DNase, RNase, protease, none detected
SMILES string
O.O.Cl[Ca]Cl
InChI
1S/Ca.2ClH.2H2O/h;2*1H;2*1H2/q+2;;;;/p-2
InChI key
LLSDKQJKOVVTOJ-UHFFFAOYSA-L
Application
Calcium chloride dihydrate has been used:
to prepare competent E. coli for transformation(118)
in the preparation of calcium polyphosphate nanospheres and to induce alkaline phosphatase(119)
as a component of renaturation buffer for the zymogram of Staphylococcus aureus crude enzyme extracts(120)
to prepare competent E. coli for transformation(118)
in the preparation of calcium polyphosphate nanospheres and to induce alkaline phosphatase(119)
as a component of renaturation buffer for the zymogram of Staphylococcus aureus crude enzyme extracts(120)
Biochem/physiol Actions
Calcium chloride is used in the heat-shock transformation of microbes with the plasmid. The positive charged calcium forms a cation bridge with phosphate of DNA and negative charged phosphorylated lipid A in lipopolysaccharide (LPS) aiding the DNA transformation into the microbes.
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signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2
Storage Class
13 - Non Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
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