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A431 Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 85090402, human skin, Epithelial

Synonym(s):

A-431 Cells, A431/P Cells

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About This Item

UNSPSC Code:
41106514

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Product Name

A431 Cell Line human, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 85090402, human skin, Epithelial

biological source

human skin

packaging

tube of 5 μg 85090402-DNA-5UG
pkg of vial of cells 85090402-1VL

growth mode

Adherent

karyotype

Hypertriploid

morphology

Epithelial

products

Not specified

receptors

Epidermal growth factor (EGF)

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Related Categories

Application

A431 Cell Line human has been used:
  • to demonstrate that p38 mitogen-activated protein kinase enhances epidermal growth factor-induced apoptosis in A431 cells
  • to study melanoma-specific CD44 (a tumor marker and transmembrane receptor) alternative splicing pattern in A431 cells
  • to study the anti-cancer activity of antioxidant-rich Cameroonian medicinal plants
Cell cycle studies

Biochem/physiol Actions

Derived from an epidermal carcinoma of the vulva taken from an 85 yr old female. The cells carry large numbers of EGF binding sites and is an indicator cell for anti-TGF binding.
Human squamous carcinoma

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Preparation Note

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

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Articles

Dynamic Live Cell Imaging of Caspase Mediated Apoptosis and Cellular Hypoxia in Lymphocytes and Cancer Cells Using the CellASIC® ONIX2 Microfluidic Platform

Application note on how the CellASIC® ONIX2 microfluidic system can be used to analyze caspase-3 mediated apoptosis/cell death and cellular hypoxia in live immune and cancer cell lines.

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