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Merck

D1556

OptiPrep™ Density Gradient Medium

used for cell and subcellular organelle isolation

Synonym(s):

Iodixanol solution

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About This Item

CAS Number:
92339-11-2
NACRES:
NA.75
PubChem Substance ID:
329798703
UNSPSC Code:
12352207
MDL number:
MFCD00867965

Key Documents

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InChI

1S/C35H44I6N6O15/c1-13(52)46(30-26(38)20(32(59)42-3-15(54)9-48)24(36)21(27(30)39)33(60)43-4-16(55)10-49)7-19(58)8-47(14(2)53)31-28(40)22(34(61)44-5-17(56)11-50)25(37)23(29(31)41)35(62)45-6-18(57)12-51/h15-19,48-51,54-58H,3-12H2,1-2H3,(H,42,59)(H,43,60)(H,44,61)(H,45,62)

SMILES string

CC(=O)N(CC(O)CN(C(C)=O)c1c(I)c(C(=O)NCC(O)CO)c(I)c(C(=O)NCC(O)CO)c1I)c2c(I)c(C(=O)NCC(O)CO)c(I)c(C(=O)NCC(O)CO)c2I

InChI key

NBQNWMBBSKPBAY-UHFFFAOYSA-N

sterility

sterile

form

liquid

density

1.319-1.321 g/mL

application(s)

hematology
histology

storage temp.

room temp

Quality Level

200

Related Categories

General description

OptiPrep™ Density Gradient Medium, also known as iodixanol, is a low osmolarity, non-ionic iodinated density gradient medium. It is a sterile and endotoxin-tested solution that comprises 60% (w/v) iodixanol in water, designed for the in vitro isolation of biological particles. It may be used for fractionation and separation of cell and subcellular organelles.

Application

OptiPrep™ has been used to isolate molecules, cells, and subcellular organelles, including DNA, cytokines, lysosomes, endoplasmic reticulum, peroxisomes, T lymphocytes, and natural killer cells. It has also been used for the following applications:

  • to investigate the efficiency of single-cell encapsulation.
  • the gradient-based isolation of senescent cancer cells.
  • isolation of non-enveloped virion isolation from bronchoalveolar lavage.
  • isolation of lipoproteins and macromolecules
  • purification of virions
  • purification of neuron cells

Features and Benefits

OptiPrep™ has numerous biocompatible properties that make it ideal for use.
  • It is biologically inert and does not interfere with assays for marker enzymes.
  • It is non-ionic, non-toxic, and has low osmotic pressure.
  • It forms spontaneous gradients that are stabler and easier to prepare than sucrose.

Other Notes

60% (w/v) solution of iodixanol in water (sterile).

Legal Information

OptiPrep is a trademark of Serumwerk Bernburg AG

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
BCCH1628
MKCQ8495
MKCQ8494
BCCG2789
BCCG3620

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Eloise Hudry et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 18(1), 44-53 (2009-08-06)
The development of Alzheimer's disease (AD) is closely connected with cholesterol metabolism. Cholesterol increases the production and deposition of amyloid-beta (Abeta) peptides that result in the formation of amyloid plaques, a hallmark of the pathology. In the brain, cholesterol is
WU Polyomavirus in Respiratory Epithelial Cells from Lung Transplant Patient with Job Syndrome
Erica A. S, et al.
Emerging Infectious Diseases, 21(1), 103-106 (2015)
Tobias Boothe et al.
eLife, 6 (2017-07-15)
In light microscopy, refractive index mismatches between media and sample cause spherical aberrations that often limit penetration depth and resolution. Optical clearing techniques can alleviate these mismatches, but they are so far limited to fixed samples. We present Iodixanol as
Tanner C Fadero et al.
eLife, 6 (2017-09-05)
Iodixanol provides an easy and affordable solution to a problem that has limited resolution and brightness when imaging living samples.
Kiyoko Okamoto et al.
Journal of virology, 82(17), 8349-8361 (2008-06-20)
Hepatitis C virus (HCV) core protein has shown to be localized in the detergent-resistant membrane (DRM), which is distinct from the classical raft fraction including caveolin, although the biological significance of the DRM localization of the core protein has not
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