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D4551

N,N-Dimethylformamide

Name: <I>N,N</I>-Dimethylformamide
Brand: SIGMA

Molecular Biology, ≥99%

Synonym(s):

DMF, NSC 5356

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About This Item

Linear Formula:

HCON(CH₃)₂

CAS Number:

68-12-2

Molecular Weight:

73.09

UNSPSC Code:

12352111

NACRES:

NA.52

PubChem Substance ID:

24893883

EC Number:

200-679-5

Beilstein/REAXYS Number:

605365

MDL number:

MFCD00003284

eCl@ss:

39031202

Assay:

≥99%

Grade:

Molecular Biology

Bp:

153 °C (lit.)

Vapor pressure:

2.7 mmHg ( 20 °C)

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Properties

grade

Molecular Biology

Quality Level

200

vapor density

2.5 (vs air)

vapor pressure

2.7 mmHg ( 20 °C)

assay

≥99%

form

liquid

autoignition temp.

833 °F

expl. lim.

15.2 %

impurities

≤0.5% water
water

refractive index

n20/D 1.430 (lit.)

bp

153 °C (lit.)

mp

−61 °C (lit.)

density

0.944 g/mL (lit.)

suitability

suitable for solvent for chromogenic substrates (X-Gal, etc.) used in molecular biology applications.

storage temp.

room temp

SMILES string

[H]C(=O)N(C)C

InChI

1S/C3H7NO/c1-4(2)3-5/h3H,1-2H3

InChI key

ZMXDDKWLCZADIW-UHFFFAOYSA-N

Description

General description

N, N-Dimethylformamide (DMF) is a solvent with a low evaporation rate, useful for preparing solutions with a variety of hydrophobic organic compounds used in molecular biology applications.

Application

Solvent for many hydrophobic organic compounds.

N,N-Dimethylformamide was used to solubilize MTT crystals in cell viability assays. It was also used in feruloyl esterase activity assay in molds exhibiting high activity of the enzyme.

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pictograms

GHS02,GHS08,GHS07

signalword

Danger

hcodes

H226,H312 + H332,H319,H360D

pcodes

P210 - P280 - P303 + P361 + P353 - P304 + P340 + P312 - P305 + P351 + P338 - P308 + P313

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Eye Irrit. 2 - Flam. Liq. 3 - Repr. 1B

Storage Class

3 - Flammable liquids

flash_point_f

135.5 °F - closed cup

flash_point_c

57.5 °C - closed cup

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

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