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D6883

2′,7′-Dichlorodihydrofluorescein diacetate

Name: 2&#8242;,7&#8242;-Dichlorodihydrofluorescein diacetate
Brand: SIGMA

≥97% (HPLC), powder, cell-permeable non-fluorescent probe

Synonym(s):

2′,7′-Dichlorofluorescin diacetate, DCFH-DA, 2′,7′-Dichlorodihydrofluorescein diacetate

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About This Item

Empirical Formula (Hill Notation):

C₂₄H₁₆Cl₂O₇

CAS Number:

4091-99-0

Molecular Weight:

487.29

NACRES:

NA.77

PubChem Substance ID:

24894058

UNSPSC Code:

12352200

MDL number:

MFCD00128955

Beilstein/REAXYS Number:

4610273

Assay:

≥97%

Form:

powder

Quality level:

200

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Properties

Product Name

2′,7′-Dichlorodihydrofluorescein diacetate, ≥97%

Quality Level

200

assay

≥97%

form

powder

storage temp.

−20°C

SMILES string

CC(=O)Oc1cc2Oc3cc(OC(C)=O)c(Cl)cc3C(c2cc1Cl)c4ccccc4C(O)=O

InChI

1S/C24H16Cl2O7/c1-11(27)31-21-9-19-15(7-17(21)25)23(13-5-3-4-6-14(13)24(29)30)16-8-18(26)22(32-12(2)28)10-20(16)33-19/h3-10,23H,1-2H3,(H,29,30)

InChI key

PXEZTIWVRVSYOK-UHFFFAOYSA-N

Description

General description

2′,7′-Dichlorodihydrofluorescein diacetate (DCFH-DA) is an oxidation-sensitive fluorescent dye used to detect the intracellular reactive oxygen species (ROS) level. DCFH-DA is a cell-permeable non-fluorescent probe, that can be hydrolyzed by intracellular esterase to DCFH, which forms fluorescent DCF when oxidized by the intracellular ROS.

The excitation wavelength is 504 nm and emission wavelength is 529 nm in ethanol. The use of DMSO as the solvent has not been determined, however various publications suggest that it is suitable.

Application

2′,7′-Dichlorofluorescin diacetate is suitable for use in:

sensitive and rapid quantitation of oxygen-reactive species in response to oxidative metabolism
microplate assay for detecting oxidative products in phagocytic cells
quantitative multiwell myeloid differentiation assay

Applications include sensitive and rapid quantitation of oxygen-reactive species in response to oxidative metabolism; microplate assay for detecting oxidative products in phagocytic cells, and quantitative multiwell myeloid differentiation assay.