P8038
Proteinase, bacterial
Type XXIV, 7.0-14.0 units/mg solid, lyophilized powder
Synonym(s):
Proteinase from Bacillus licheniformis, Alkaline Protease, Protease from Bacillus licheniformis, Subtilo peptidase A
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About This Item
CAS Number:
UNSPSC Code:
12352204
eCl@ss:
32160410
EC Number:
232-752-2
NACRES:
NA.54
MDL number:
Specific activity:
≤0.05 Kunitz units/mg solid RNase, ≤5.0 Kunitz units/mg solid DNase, 7.0-14.0 units/mg solid
type
Type XXIV
form
lyophilized powder
specific activity
≤0.05 Kunitz units/mg solid RNase, ≤5.0 Kunitz units/mg solid DNase, 7.0-14.0 units/mg solid
mol wt
27 kDa
purified by
crystallization
shipped in
wet ice
storage temp.
−20°C
Quality Level
Related Categories
General description
Subtilisin is a non-glycosylated single polypeptide chain without disulfide bonds and has a molecular weight of 27 KDa.
Application
The enzyme from Sigma has been used to optimize release of all mitochondrial populations from homogenized ventricular tissue of rat heart. It has also been used in the pre-hybridisation treatment of formalin fixed, paraffin wax-embedded liver specimens for detecting human and viral DNA.
This is a proteolytic enzyme isolated from the fermentation of Bacillus licheniformis. It is a serine endoproteinase with a broad specificity towards native and denatured proteins, and is active under alkaline conditions. Product P8038, also known as Subtilisin Carlsberg, has been used to hydrolyze cardiac cells to study the silencing of cardiac mitochondrial NHE1.
Biochem/physiol Actions
Subtilisin A is a member of the Serine S8 Endoproteinase family. It has broad specificity with a preference for a large uncharged residue in the P1 position. It hydrolyzes native and denatured proteins, and is active under alkaline conditions.
Proteinase catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate .
Analysis Note
Amino acid analysis and isoelectric focusing electrophoresis consistent with subtilisin Carlsberg.
Other Notes
One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).
signalword
Danger
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
10 - Combustible liquids
wgk
WGK 2
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Journal of clinical pathology, 41(7), 793-798 (1988-07-01)
A rapid and reproducible technique for in situ hybridisation, using biotin labelled probes for the Y chromosome, human DNA, hepatitis B virus DNA and cytomegalovirus DNA on formalin fixed, paraffin embedded liver tissue, was developed. The degree of proteolytic digestion
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The Journal of biological chemistry, 262(16), 7859-7864 (1987-06-05)
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