Skip to Content
Merck

PPC1010

Protease and Phosphatase Inhibitor Cocktail

DMSO solution, for the inhibition of serine, cysteine, acid proteases, aminopeptidase, serine/threonine protein phosphatases and L-isozymes of alkaline phosphatase

Synonym(s):

Protease and Phosphatase Inhibitor Mix, Protease and phosphatase inhibitor

Sign In to View Organizational & Contract Pricing.

Select a Size

About This Item

UNSPSC Code:
12352200
NACRES:
NA.54
Form:
DMSO solution
Solubility:
water: soluble
Storage temp.:
−20°C

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Product Name

Protease and Phosphatase Inhibitor Cocktail,

form

DMSO solution

Quality Level

200

packaging

pkg of 1 ml, 5 ml ×

solubility

water: soluble

shipped in

wet ice

storage temp.

−20°C

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

Protease and Phosphatase Inhibitor Cocktail is designed to increase the yield of intact and unmodified proteins in cell extracts. Protease and Phosphatase Inhibitor cocktail mixture inhibits serine, cysteine, and acid proteases, as well as aminopeptidase. It also inhibits serine/threonine protein phosphatases and L-isozymes of alkaline phosphatase. The cocktail has been tested on extracts from various animal tissues such as the human placenta and bovine liver and on extracts of A431, CHO, and U937 cells. Protease and Phosphatase Inhibitor Cocktail is supplied as a ready-to-use solution in DMSO.

Application

Protease and Phosphatase Inhibitor Cocktail has been used as a supplement in radioimmunoprecipitation assay (RIPA) buffer:
  • for cellular protein extraction
  • to lyse purified CD4+ T cells
  • to prepare protein extracts from placental tissues and cells

Protease and Phosphatase Inhibitor Cocktail has been used in protein isolation buffers.

Preparation Note

The recommended dilution of the cocktail in the biological extract is 1 ml of the cocktail per 500 mg of protein extracted from tissue or cells. In many cases, the cocktail can be used at a final concentration of 1% (v/v), 1 ml of cocktail solution per 100 ml of extraction buffer.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research
This product is for R&D use only, not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H315,H319

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

188.6 °F

flash_point_c

87 °C

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Marcela Rassi-Cruz et al.
Endocrine-related cancer, 28(1), 1-13 (2020-10-29)
Familial primary aldosteronism (PA) is rare and mostly diagnosed in early-onset hypertension (HT). However, 'sporadic' bilateral adrenal hyperplasia (BAH) is the most frequent cause of PA and remains without genetic etiology in most cases. Our aim was to investigate new
E Zoico et al.
Mechanisms of ageing and development, 192, 111374-111374 (2020-10-06)
Fibrosis has been considered as a hallmark of dysfunctional adipose tissue (AT), however the role and mechanisms of fibrosis in the age related AT dysfunction are not yet well characterized. The aim of the study was to investigate the mechanisms
Beta-Arrestin1 Prevents Preeclampsia by Downregulation of Mechanosensitive AT1-B2 Receptor Heteromers
Quitterer U, et al.
Cell, S0092-8674(18), 31406-31406 (2018)
Sylwia Dabrowska et al.
Experimental neurology, 336, 113531-113531 (2020-11-23)
Stroke is the leading cause of long-term, severe disability worldwide. Immediately after the stroke, endogenous inflammatory processes are upregulated, leading to the local neuroinflammation and the potentiation of brain tissue destruction. The innate immune response is triggered as early as
Graphene quantum dots prevent alpha-synucleinopathy in Parkinson?s disease
Kim D, et al.
Nature Nanotechnology, 13(9), 812-812 (2018)
View All Related Papers

Related Content

Data Sheet - PPC1010
용해 및 단백질 추출

세포 용해 및 단백질 추출 방법은 세제 용해부터 기계적 파쇄법에 이르기까지 다양한 기술을 포함하며 연구에 필요한 요구 사항을 지원합니다.

Lysis & Protein Extraction

Cell lysis and protein extraction methods overview various techniques, from detergent solubilization to mechanical disruption, supporting research needs.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service