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Merck

199303

Resazurin sodium salt

certified by the BSC

동의어(들):

7-Hydroxy-3H-phenoxazin-3-one-10-oxide sodium salt

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크기 선택


제품정보 (DICE 배송 시 비용 별도)

실험식(Hill 표기법):
C12H6NNaO4
CAS 번호:
Molecular Weight:
251.17
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
EC Number:
263-718-5
Beilstein/REAXYS Number:
3794971
MDL number:
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InChI key

IVGPGQSSDLDOLH-UHFFFAOYSA-M

InChI

1S/C12H7NO4.Na/c14-7-1-3-9-11(5-7)17-12-6-8(15)2-4-10(12)13(9)16;/h1-6,14H;/q;+1/p-1

SMILES string

[Na+].[O-]c1ccc2c(OC3=CC(=O)C=CC3=[N+]2[O-])c1

agency

certified by the BSC

form

powder

composition

Dye content, 75%

solubility

1 M NH4OH: 1 mg/mL

λmax

380 nm (2nd), 598 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

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General description

Resazurin is a small N-oxide derivative of dihydroxyphenoxazone. It is a non-ionic dye containing a small and planar chromophore. It is a redox indicator and changes from bluish violet through pink to colorless upon reduction. It is also used as a pH indicator for Ph 3.5 to 6.5 wherein it changed from orange to bluish violet. It is commercially available as resazurin sodium salt, which is also known as 3H-Phenoxazin-3-one, 7-hydroxy-, 10-oxide, sodium salt. It forms the red, fluorescent hydroxyphenoxazone resorufin on reduction and colorless hydroresorufin on further reduction.

Application

For the measurement of metabolic activity and proliferation of living cells. The bioreduction of the dye reduces the amount of the oxidized form (blue) and concomitantly increases the fluorescent intermediate (red).
Resazurin is mostly used as a biological redox indicator. The bioreduction of the dye reduces the amount of the oxidized form (blue) and concomitantly increases the fluorescent intermediate (red). Its applications include:
  • the measurement of metabolic activity and the proliferation of living cells
  • the detection of bacterial reduction in milk
  • in viability assays as a component of culture media for the detection of viable bacteria
  • to determine the number of motile sperm in sheep semen
  • to assay lactate dehydrogenase
  • as an analytical reagent to quantify L-glutamate

pictograms

Exclamation mark

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

저장 등급

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves


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