539134
Protease Inhibitor Cocktail III
EDTA-Free, liquid, for the inhibition of aspartic, cysteine, and serine proteases and aminopeptidases in mammalian cells and tissues.
동의어(들):
EDTA free Protease inhibitor, EDTA-Free Protease Inhibitor Mix, Protease inhibitor cocktail III
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크기 선택
제품정보 (DICE 배송 시 비용 별도)
UNSPSC Code:
12352200
NACRES:
NA.77
Form:
liquid
Solubility:
water: soluble
Storage temp.:
−20°C
제품 이름
Protease Inhibitor Cocktail Set III, EDTA-Free, Protease inhibitor cocktail III, EDTA-free for inhibiting aspartic, cysteine, and serine proteases as well as aminopeptidases in mammalian cells and tissues.
form
liquid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
solubility
water: soluble
shipped in
wet ice
storage temp.
−20°C
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General description
Note: available as 1 x 1 ml or as a set of 5 x 1 ml.
This protease inhibitor cocktail set is a specially formulated cocktail of six protease inhibitors with broad specificity for the inhibition of aspartic, cysteine, and serine proteases as well as aminopeptidases. This cocktail is recommended for use with mammalian cells and tissue extracts, but is also suitable for bacterial extracts. It is provided as a single vial or as a set of 5 vials. Each vial contains 1 ml protease inhibitor cocktail solubilized in DMSO with a small amount of water.
Application
Protease Inhibitor Cocktail Set III, EDTA-Free has been used:
- as a component in CelLytic MT cell lysis reagent or lysis buffer to homogenize kidney organoids for immunoblot analysis
- as a component in homogenization buffer to homogenize minced mouse quadriceps femoris muscle tissue
- in lysis buffer to extract proteins for immunoblotting
Biochem/physiol Actions
Inhibits a broad spectrum of serine proteases, cysteine proteases, and aspartic proteases, as well as aminopeptidases.
Protease inhibitor cocktails and specialized protease inhibitors play a crucial role in various proteomic studies conducted by scientists. These studies encompass protein expression and characterization, biomarker discovery, identification of post-translational modifications, protein expression profiling, and quantitative protein measurement. The inclusion of a protease inhibitor cocktail or specific protease inhibitor guarantees the preservation of protein integrity, allowing for accurate downstream analysis and comprehensive sample characterization.
Features and Benefits
- EDTA-Free: recommended for IMAC applications.
- Flexible: compatible with bacterial extracts, mammalian cell extracts, and tissue extracts.
- Fast: no waiting time for tablets to dissolve.
- Economical: ready-to-use format reduces wastage.
- Effective: one ml is sufficient for approximately 20g of tissue.
- Complete: contains six protease inhibitors to inhibit aspartic, cysteine, serine proteases, and aminopeptidases.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Irritant (B)
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2
저장 등급
10 - Combustible liquids
flash_point_f
188.6 °F - (refers to pure substance)
flash_point_c
87 °C - (refers to pure substance)
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Daniel S Levic et al.
The Journal of cell biology, 219(4) (2020-04-25)
Epithelial cell physiology critically depends on the asymmetric distribution of channels and transporters. However, the mechanisms targeting membrane proteins to the apical surface are still poorly understood. Here, we performed a visual forward genetic screen in the zebrafish intestine and
Olga Vasiljeva et al.
Scientific reports, 10(1), 5894-5894 (2020-04-05)
Proteases have been implicated in the development of many pathological conditions, including cancer. Detection of protease activity in diseased tissues could therefore be useful for diagnosis, prognosis, and the development of novel therapeutic approaches. Due to tight post-translational regulation, determination
Cao Chen et al.
Medical microbiology and immunology, 209(1), 81-94 (2019-11-14)
Activation of complement system in central nervous system (CNS) of the patients suffering from prion diseases or animal models infected with prion agents experimentally is reported repeatedly, but which pathways are involved in the complement system during prion infection is
Bruno E Rojas et al.
Journal of experimental botany, 72(7), 2514-2524 (2020-12-15)
Phosphoenolpyruvate carboxykinase (PEPCK) plays a crucial role in gluconeogenesis. In this work, we analyze the proteolysis of Arabidopsis thaliana PEPCK1 (AthPEPCK1) in germinating seedlings. We found that the amount of AthPEPCK1 protein peaks at 24-48 h post-imbibition. Concomitantly, we observed
Wei-Ling Wang et al.
American journal of physiology. Renal physiology, 318(4), F956-F970 (2020-02-25)
Aquaporin-2 (AQP2) is a vasopressin-regulated water channel protein responsible for osmotic water reabsorption by kidney collecting ducts. In response to vasopressin, AQP2 traffics from intracellular vesicles to the apical plasma membrane of collecting duct principal cells, where it increases water
문서
Learn tips and tricks for how to properly use inhibitors including how to select the right inhibitor and how to plan experiments with inhibitors.
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관련 콘텐츠
An Introduction to Inhibitors and Their Biological Applications
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| 539134-1SETCN | 07790788059750 |
| 539134-1MLCN | 07790788051280 |
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