제품 이름
Anti-Collagen Type I Antibody, Chemicon®, from rabbit
biological source
rabbit
antibody form
purified immunoglobulin (Semi-purified)
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable, immunohistochemistry: suitable, western blot: suitable
suitability
not suitable for immunohistochemistry (Paraffin)
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... COL1A1(1277)
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Immunogen
Human placental collagen type I
Application
ELISA on human collagen type I: 1:4,000.
Indirect microimmunofluorescent visualization of collagen type I on cryostat sections of human tissue or cultured cells: 1:10-1:40.
Western blot 1:200
Not recommended for use in paraffin treated tissues.
Optimal working dilutions must be determined by the end user.
Indirect microimmunofluorescent visualization of collagen type I on cryostat sections of human tissue or cultured cells: 1:10-1:40.
Western blot 1:200
Not recommended for use in paraffin treated tissues.
Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
ECM Proteins
ECM Proteins
This Anti-Collagen Type I Antibody is validated for use in ELISA, IH, WB for the detection of Collagen Type I.
Biochem/physiol Actions
Recognizes Human collagen type I. Some cross reactivity with other proteins.
Physical form
Ammonium sulfater precipitation followed by DEAE chromatography, and immunodeletion
Format: Semi-Purified
Immunoglobulin fraction, prepared by ammonium sulfate precipitation and chromatography on DEAE-cellulose, of antiSerum cross-absorbed over immobilized human albumin, immunoglobulin and collagen types III, IV and V.
Product is liquid in 0.15M Sodium Chloride, 10mM Sodium Phosphate pH 7.5 with 0.1% Mannitol and 0.1% Dextran as stabilizers. No preservative.
Product is liquid in 0.15M Sodium Chloride, 10mM Sodium Phosphate pH 7.5 with 0.1% Mannitol and 0.1% Dextran as stabilizers. No preservative.
Preparation Note
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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저장 등급
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
In vitro enhancement of collagen matrix formation and crosslinking for applications in tissue engineering: a preliminary study.
Ricky R Lareu, Irma Arsianti, Harve Karthik Subramhanya, Peng Yanxian, Michael Raghunath
Tissue Engineering null
Matthias Schieker et al.
Journal of anatomy, 204(2), 133-139 (2004-03-23)
In stem-cell research a major difficulty is caused by the lack of distinctive features that allow the identification of human mesenchymal stem cells (hMSC). Until now, there has been no specific marker and the most common way to identify hMSC
M Fairuz B Jamaluddin et al.
Endocrinology, 159(7), 2656-2669 (2018-05-23)
Uterine leiomyomas (fibroids) are the most common benign tumors that are associated with increased production of extracellular matrix (ECM). Excessive ECM deposition plays a major role in the enlargement and stiffness of these tumors and contributes to clinical symptoms, such
In situ expression of connective tissue growth factor in human crescentic glomerulonephritis.
Katsuyoshi Kanemoto, Joichi Usui, Kosaku Nitta, Shigeru Horita, Atsumi Harada et al.
Virchows Archiv null
E Castaños-Velez et al.
Immunology, 86(2), 270-278 (1995-10-01)
The extracellular matrix (ECM) proteins collagen I, III and IV, laminin, fibronectin, vitronectin, thrombospondin, tenascin and their integrin receptors of the beta 1 and beta 3 subfamilies showed characteristic patterns of distribution in different compartments of non-reactive and reactive lymph
프로토콜
TrueGel3D® Hydrogel Plate protocol guides high-throughput culture of human adipose MSCs for screening applications.
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| AB745 | 04053252510786 |
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