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Merck

ESG1107

ESGRO® Recombinant Mouse LIF Protein

ESGRO Leukemia Inhibitory Factor (LIF) supplement for mouse ES cell culture. Each vial contains 10^7 units/ml.

동의어(들):

Mouse LIF, Mouse Leukemia Inhibitory Factor, Murine LIF, Murine LIF, Mouse LIF, mLIF, mLIF

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제품정보 (DICE 배송 시 비용 별도)

UNSPSC Code:
12352202
NACRES:
NA.75
eCl@ss:
32160801
Form:
liquid
Assay:
>95% (active component mLIF, SDS-PAGE)

주요 문서

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도움 문의

assay

>95% (active component mLIF, SDS-PAGE)

form

liquid

manufacturer/tradename

Chemicon®

concentration

10000000 units/mL

technique(s)

cell culture | stem cell: suitable

input

sample type induced pluripotent stem cell(s)
sample type: mouse embryonic stem cell(s)

NCBI accession no.

NM_008501

UniProt accession no.

P09056

shipped in

wet ice

Quality Level

100

Gene Information

mouse ... LIF(16878)

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관련 카테고리

General description

Mouse Leukemia Inhibitory Factor (LIF, mLIF) promotes self-renewal and long-term maintenance of pluripotent mouse embryonic stem (ES) and induced pluripotent stem (iPS) cells by suppressing spontaneous differentiation.
The ESGRO mouse LIF medium supplement is supplied in active units/mL for consistent performance from lot to lot. Each lot of ESGRO supplement undergoes stringent quality control testing to ensure reliable inhibition of mouse pluripotent stem cell differentiation. ESGRO mouse LIF supplement may be used for feeder based and feeder free culture of mouse embryonic stem (ES) and induced pluripotent stem (iPS) cells.

Application

Applications for ESGRO Supplement include use as a reagent for the in vitro maintenance of the pluripotential phenotype of murine ES cells.

SUGGESTED PROTOCOLS:

ES Cells: In D3 and MBL-1 pluripotential ES cell cultures it is routinely found that 1000 U ESGRO Supplement per 1.0 mL of tissue culture media is required to maintain ES cells with a stem cell phenotype. Similar concentrations of mLIF have also been used for germline transmission of genetically altered ES cells(E3).

At the recommended concentration 107 units of ESGRO is sufficient for 10.0 L of tissue culture media and 106 units of ESGRO Supplement is sufficient for 1.0 L of tissue culture media.

Physical form

ESGRO Supplement is supplied in liquid form as 107 Units in 1.0 mL of phosphate buffered saline with 1% w/v bovine serum albumin BSA as a carrier for stability.
Format: Purified

Preparation Note

ESGRO® Supplement is supplied 0.22 micron sterile filtered, and tested negative in mycoplasma tests.
ESGRO Supplement is shipped at ambient temperature. A cold pack may be included for customer relations, however it is truly not needed. Extensive stability tests have been performed with ESGRO Supplement during development. This product is stable for at least 18 months from the date of manufacture, in the concentrated form or diluted in sterile tissue culture media, with no loss of activity on ES cells. For long term storage it is recommended that ESGRO Supplement be stored at 4°C. Freeze-thawing will reduce potency. It is recommended that prior to use, ESGRO Supplement should be diluted in sterile tissue culture media and aliquoted to a convenient concentration, then stored at 4°C. Freeze-thawing should be avoided. Product is stable for a minimum of 7 days at 37°C, 5% CO2 incubator during the culture of ES cells.

Analysis Note

Specific Activity: ESGRO® Supplement is assessed both on mouse embryonic stem cells (D3 & MBL-1) and on murine M1 myeloid leukemic cells(4). A standard of 50 Units is defined as the concentration of ESGRO Supplement, in 1.0mL of tissue culture medium, that induces the differentiation of 50% of M1 colonies(4). 10E7 units is equivalent to approximately 100 μg of pure protein, and is sufficient to treat 10L of ES cell culture media.

Embryonic Stem Cell Assay: Differentiation inhibition at 1000 units/mL Murine myeloid leukemic, M1 Assay: Specific Activity >/= 108 units/mg

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
ESGRO is a registered trademark of Merck KGaA, Darmstadt, Germany

저장 등급

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Xudong Fu et al.
Science advances, 6(22), eaay5181-eaay5181 (2020-06-12)
In mouse embryonic stem cell (ESC), a small cell population displays totipotent features by expressing a set of genes that are transiently active in 2-cell-stage embryos. These 2-cell-like (2C-like) cells spontaneously transit back into the pluripotent state. We previously dissected
Isolation and characterization of neural crest-derived stem cells from dental pulp of neonatal mice.
Janebodin, Kajohnkiart, et al.
Testing, 6, e27526-e27526 (2011)
Novel hyperactive transposons for genetic modification of induced pluripotent and adult stem cells: a nonviral paradigm for coaxed differentiation.
Belay, Eyayu, et al.
Stem Cells, 28, 1760-1771 (2010)
Dynamic regulation of 5-hydroxymethylcytosine in mouse ES cells and during differentiation.
Ficz G. et al.
Nature null
Proliferating versus differentiating stem and cancer cells exhibit distinct midbody-release behaviour.
Ettinger A. W. et al.
Nature Communications null
모든 관련된 서류 보기

문서

Leukemia Inhibitory Factor (LIF) Protein

Leukemia Inhibitory Factor (LIF) is a stem cell growth factor used for the in vitro culture of pluripotent mouse embryonic stem cells (ES cells).

Naive Pluripotent Stem Cell Culture in Serum-Free Media

Naive pluripotent stem cells cultured in vitro using specialized media and inhibitors mimic "ground-state" cells from blastocysts.

Pluripotent and Multipotent Stem Cells

Read types of stem cells including multipotent stem cells, Pluripotent stem cells and iPSCs and their applications in basic stem cell research, stem cell therapy and disease modelling

프로토콜

Mouse Embryonic Stem Cell Culture Procedures & Protocols

Stem Cell protocols for cryopreservation, thawing of cryopreserved stem cells and media preparation.

관련 콘텐츠

ES Cell Culture using ESGRO Medium Supplement
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국제 무역 품목 번호

SKUGTIN
ESG110708436037124310

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