Anti-CSPG4 Antibody

Human M14 melanoma cell extract depleted of fibronectin and bound by lens culinaris lectin-Sepharose (Morgan et al., 1981).

Detect Chondroitin Sulfate Proteoglycan using this Anti-Chondroitin Sulfate Proteoglycan Antibody, clone 9.2.27 validated for use in FC, IF & IP.

Immunofluorescence microscopy of cells in culture (Bumoi et al., 1984; Schrappe et al., 1992). Also, Legg J et al (2003) Development 130:6049-6063 {3% PFA fixed frozen sections}.

Immunoelectron microscopy (Bumoi et al., 1984)

Immunoprecipitation: 1μg/10E6 cells (Bumoi et al., 1984; Morgan et al., 1981)

FACS analysis: 1-2μg/10E6 cells

Functional block of melanoma cell spreading (Morgan et al., 1981), glioma cell proliferation3 and tumor growth in nude mice (Morgan et al., 1981; Schrappe et al., 1992).

Optimal working dilutions must be determined by end user.

Research Category
Cell Structure

Research Sub Category
ECM Proteins

Recognizes mature chondroitin sulfate proteoglycan core glycoprotein of 250 kDa as well as precursor polypeptides of 210, 220 and 240 kDa (Bumoi et al., 1984). Reacts with human melanoma, glioma and proliferating brain endothelial cells.

Format: Purified

Purified immunoglobulin in 0.02M PBS pH 7.6, 0.25M NaCl containing 0.1% sodium azide.

Maintain between 2 and 8°C in undiluted aliquots for up to 6 months.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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