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Merck

MAB3408

Anti-β-Tubulin III Antibody

CHEMICON®, mouse monoclonal, KMX-1

동의어(들):

Tubulin beta-III, tubulin, beta 3, tubulin, beta 4

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제품정보 (DICE 배송 시 비용 별도)

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Clone:
KMX-1, monoclonal
Species reactivity:
human, rat, mouse
Application:
ICC, WB
Technique(s):
immunocytochemistry: suitable, western blot: suitable
Citations:
172
Uniprot accession no.:
Q13509

주요 문서

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제품 이름

Anti-Tubulin Antibody, beta, clone KMX-1, clone KMX-1, Chemicon®, from mouse

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

KMX-1, monoclonal

species reactivity

human, rat, mouse

species reactivity (predicted by homology)

all

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable, western blot: suitable

isotype

IgG2b

NCBI accession no.

NM_006086.2

UniProt accession no.

Q13509

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... TUBB3(10381)

Physical form

Purified mouse monoclonal IgG2b liquid in buffer containing 0.02M phosphate buffer, 0.25M NaCl with 0.1% sodium azide.
Format: Purified
Protein A purified

Preparation Note

Stable for 6 months at 2-8ºC in undiluted aliquots from date of receipt.

Analysis Note

Control
HeLa Cell lysate

A431 Cell lysate

MCF7 Cell lysate
Routinely evaluated by Western Blot on A431 lysates.

Western blot:
1:500 dilution of this lot detected TUBULIN (BETA) on 10 μg of A431 lysates.

Application

Immunocytochemistry:
A 1-2 μg/mL concentration of a previous lot was used in immunocytochemistry.

Western blot:
0.05-0.5 μg/mL

Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Research Sub Category
Cytoskeleton
This Anti-Tubulin Antibody, beta, clone KMX-1 is validated for use in IC, WB for the detection of Tubulin.

Biochem/physiol Actions

This antibody reacts specifically with β-tubulin and shows a strong cross-reactivity to different species. This antibody allows staining of microtubuli using indirect immunofluorescence showing a low background only.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

50 kDa
Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 kDa. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of beta Tubulin may not be stable in certain cells. For example, expression of tubulin in adipose tissue is very low and therefore beta Tubulin should not be used as loading control for these tissues.

Immunogen

Tubulin purified from polycephalum myxamoebae

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: 04-1049

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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저장 등급

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

시험 성적서(COA)

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Inhibition of telomerase by targeting MAP kinase signaling.
Dakang Xu,He Li,Jun-Ping Liu
Methods in Molecular Biology null
Polycystin-1 is a microtubule-driven desmosome-associated component in polarized epithelial cells.
Basora, Nuria, et al.
Experimental Cell Research, 316, 1454-1464 (2010)
Contactin-1 regulates myelination and nodal/paranodal domain organization in the central nervous system.
Colako?lu, G; Bergstrom-Tyrberg, U; Berglund, EO; Ranscht, B
Proceedings of the National Academy of Sciences of the USA null
Richard J Flannery et al.
Frontiers in synaptic neuroscience, 4, 6-6 (2012-12-12)
N-cadherin is a calcium-sensitive cell adhesion molecule commonly expressed at synaptic junctions and contributes to formation and maturation of synaptic contacts. This study used heterologous cell cultures of brainstem cholinergic neurons and transfected Chinese Hamster Ovary (CHO) cells to examine
Merlin/NF2 regulates angiogenesis in schwannomas through a Rac1/semaphorin 3F-dependent mechanism.
Wong, HK; Shimizu, A; Kirkpatrick, ND; Garkavtsev, I; Chan, AW; di Tomaso, E; Klagsbrun, M; Jain, RK
Neoplasia null
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