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Merck

SCR004

Alkaline Phosphatase Detection Kit

This Alkaline Phosphatase Detection Kit is a specific & sensitive tool for the phenotypic assessment of Embryonic Stem (ES) cell differentiation by the determination of AP activity.

동의어(들):

ALP Stain, Alkaline Phosphtase Stem Cell Staining Kit, Stem Cell AP Staining Kit

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제품정보 (DICE 배송 시 비용 별도)

UNSPSC Code:
12352207
NACRES:
NA.84
eCl@ss:
32161000
기술 서비스
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도움 문의

Quality Level

species reactivity (predicted by homology)

mammals

manufacturer/tradename

Chemicon®

technique(s)

cell culture | stem cell: suitable

input

sample type cardiac stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type hematopoietic stem cell(s)
sample type induced pluripotent stem cell(s)
sample type neural stem cell(s)
sample type epithelial cells
sample type pancreatic stem cell(s)
sample type mesenchymal stem cell(s)
sample type: human embryonic stem cell(s)

shipped in

wet ice

storage temp.

2-8°C

General description

Millipore′s Alkaline Phosphatase Detection Kit (Catalog number SCR004) is a specific and sensitive tool for the phenotypic assessment of ES cell differentiation by the determination of AP activity.

Application

Embryonic stem (ES) cells are totipotent cells derived from the inner cell mass (ICM) of preimplantation mammalian embryos and are capable of unlimited, undifferentiated proliferation in vitro [1,2,3]. Undifferentiated murine ES cells can be maintained in vitro for extended periods in media containing the cytokine, leukemia-inhibitory factor (LIF) or Millipore′s proprietary ES cell culture reagent, ESGRO [4,5]. The undifferentiated state of ES cells can be characterized by high level of expression of Alkaline Phosphatase (AP) [6], the expression of surface markers including SSEA and TRA antigens and the transcription factor Oct-4.

Millipore′s Alkaline Phosphatase Detection Kit (Catalog number SCR004) is a specific and sensitive tool for the phenotypic assessment of ES cell differentiation by the determination of AP activity.

Available separately from Millipore are the monoclonal antibodies TRA-2-49 (Catalog Number MAB4349) and TRA-2-54 (Catalog Number MAB4354), which permit the detection of Liver / Bone / Kidney isozyme of AP as expressed by ES cells. When used in flow cytometry, these reagents permit a quantitative assessment of AP expression by ES cells [7].

For Research Use Only; Not for use in diagnostic procedures
Research Category
Stem Cell Research

Preparation Note

The Alkaline Phosphatase Detection Kit consists of two components used for determining AP activity.

When stored at 2-8º C, the kit components are stable up to the expiration date. Do not freeze or expose to elevated temperatures. Discard any remaining reagents after the expiration date.

Other Notes

Fast Red Violet solution (0.8g/L stock) (Part No. 90239). Two 15mL bottles.

Napthol AS-BI phosphate solution (2mg/mL) in a buffer, pH 8.5 (Part No. CS235583). Two bottles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

signalword

Warning

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

pictograms

Health hazard

hcodes

Hazard Classifications

Carc. 2

저장 등급

12 - Non Combustible Liquids


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Embryonic stem cells require Wnt proteins to prevent differentiation to epiblast stem cells.
Derk ten Berge,Dorota Kurek,Tim Blauwkamp,Wouter Koole,Alex Maas,Elif Eroglu,Ronald K Siu,Roel Nusse
Nature Cell Biology null
Establishment of LIF-dependent human iPS cells closely related to basic FGF-dependent authentic iPS cells.
Hiroyuki Hirai,Meri Firpo,Nobuaki Kikyo
Testing null
Jinkyung Cho et al.
The Journal of nutritional biochemistry, 77, 108327-108327 (2020-01-12)
Self-renewal is required for embryo stem cells (ESCs) and adipose-derived mesenchymal stem cells (ADMSCs). This study examined the ability of ferulic acid in mouse ESCs and ADMSCs, in a high fat diet-induced mouse model. Initially, five natural compounds of ferulic
Ahmed K Elsayed et al.
Stem cell research, 42, 101705-101705 (2020-01-29)
FOXA2 is a transcription factor, playing an important role during development. We established an induced pluripotent stem cell (iPSC) line, QBRIi009-A, using non-integrating Sendai virus from a 4-year-old boy, displaying a complex clinical phenotype. Molecular karyotyping and cytogenetics confirmed a
Efficient iPS cell production with the MyoD transactivation domain in serum-free culture.
Hiroyuki Hirai,Nobuko Katoku-Kikyo,Peter Karian,Meri Firpo,Nobuaki Kikyo
Testing null

문서

Read types of stem cells including multipotent stem cells, Pluripotent stem cells and iPSCs and their applications in basic stem cell research, stem cell therapy and disease modelling

프로토콜

This page covers the indirect co-culture of embryonic stem cells with embryonic fibroblasts.

Step-by-step stem cell culture protocols for human induced pluripotent stem cells (iPSCs) including ips cell thawing, expanding, freezing and characterizing.

iPS 세포 해동, 증식, 냉동, 특성화를 포함한 인간 유도만능줄기세포(iPSC)를 위한 줄기세포 배양의 단계별 프로토콜.

관련 콘텐츠

As the focus of stem cell research undergoes a transition from animal to human models, researchers are in critical need of validated products to support the isolation, maintenance, differentiation, and characterization of human stem cells. While many reagents designed for rodent systems can be applied to human stem cell studies, they are not truly optimized for robust human stem cell culture or analysis. This is why human stem cell researchers have always trusted EMD Millipore, the first provider of commercially available human embryonic stem cells and human neural stem cell lines, to accelerate their research. All of our human stem cell systems are extensively tested in defined media culture, and differentiated progeny are comprehensively characterized with highly validated antibodies and detection reagents.

We have collaborated with Plasticell Ltd. to develop OsteoMAX-XF™, a novel, serum-free, xeno-free, differentiation medium for the generation of osteoblasts from human MSCs. This medium may be particularly suitable for drug discovery and clinical development. By screening roughly 3,500 combinations of serum and xeno-free media, we discovered a remarkably efficient, fully defined, reproducible medium to promote the differentiation of hMSCs into osteoblasts, offering an excellent, cost effective method to produce large amounts of human bone cells for multiple applications.

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국제 무역 품목 번호

SKUGTIN
SCR00404053252503757

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