Anti-Digoxigenin

Digoxigenin is a hapten which is used in labeling of nucleic acids and in detection systems.

Probes labeled with digoxigenin has greater sensitivity equivalent to that of radioactive probes. It allows faster detection, is less hazardous and has an increased shelf life.

Use Anti-Digoxigenin antibody for the detection of digoxigenin-labeled compounds using:

• ELISA
• Immunohistocytochemistry
• In situ hybridization
• Western blot

Anti-Digoxigenin has been used in DNA tethering. It has been used to attach DNA molecule to the glass surface of the flow cell.

The polyclonal antibody from sheep is specific to digoxigenin and digoxin and shows no cross-reactivity with other steroids, such as human estrogens and androgens.

In the presence of Na⁺, Mg²⁺ and ATP, digoxigenin inhibits sodium pumps.

Working concentration: Working concentration of antibody depends on application and substrate. The following concentrations should be taken as a guideline:

• ELISA: for coating: 2 to 4 μg/ml
• Immunohistocytochemistry: 0.5 to 2 μg/ml
• In situ hybridization: 0.2 to 0.4 μg/ml
• Western blot: 0.5 to 2 μg/ml

Working solution: For coating applications: PBS (phosphate buffered saline), pH 7.4

Add 1 ml PBS to a final concentration of 200 μg/ml.

After immunization with digoxigenin, sheep IgG was purified by ion-exchange chromatography, and the specific IgG was isolated by immunosorption.

No cross reactivity with other steroids, such as human estrogens e.g., estradiol or androgens e.g., testosterone.

For life science research only. Not for use in diagnostic procedures.