biological source
fish (sperm)
grade
Molecular Biology
form
solution (ready-to-use)
mol wt
40-1,000 kDa
packaging
pkg of 500 mg
manufacturer/tradename
Roche
concentration
100 μg/mL
color
light yellowopalescent
solubility
water: miscible
storage temp.
−20°C
General description
DNA, MB-grade is used for the prevention of nonspecific hybridization reactions.
DNA, MB-grade, is provided as a sonicated, denatured single-stranded DNA fragment mixture.
Application
The ready-to-use solution is directly added to the hybridization mix. It prevents nonspecific hybridization in all types of membrane (e.g., Southern and northern blot applications) or in situ DNA hybridizations. DNA, MB-grade has been used in chromatin immunoprecipitation (ChiP) assay.
Note: Addition of NaCl, sonication or shearing, and denaturing are not required.
Note: Addition of NaCl, sonication or shearing, and denaturing are not required.
Physical form
Solution, 10 mg/ml, in 10 mM Tris-HCl, 10 mM NaCl, 1 mM EDTA, pH 8.0
Preparation Note
Working concentration: 100μg/ml
This fish sperm DNA should be used at a concentration of 100 μg/ml (1 : 100 dilution) in prehybridization solutions.
This fish sperm DNA should be used at a concentration of 100 μg/ml (1 : 100 dilution) in prehybridization solutions.
Other Notes
Chain Length 120 to 3,000 nucleotides
For life science research only. Not for use in diagnostic procedures.
저장 등급
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
No data available
flash_point_c
No data available