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93595

Trypan Blue solution

Name: Trypan Blue solution
Brand: SIAL

0.4%, suitable for microscopy

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제품정보 (DICE 배송 시 비용 별도)

실험식(Hill 표기법):

C₃₄H₂₄N₆Na₄O₁₄S₄

CAS 번호:

72-57-1

Molecular Weight:

960.81

UNSPSC Code:

12171500

NACRES:

MA.02

PubChem Substance ID:

329770254

MDL number:

MFCD00003969

Beilstein/REAXYS Number:

4360496

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속성

form

liquid

Quality Segment

200

shelf life

limited shelf life, expiry date on the label

concentration

0.4%

technique(s)

microbe id | staining: suitable

color

blue to very dark blue

density

1.007 g/mL at 20 °C

ε_max

1.4 at 603 nm in methanol

suitability

suitable for microscopy

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4

InChI

1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4

InChI key

GLNADSQYFUSGOU-UHFFFAOYSA-J

설명

General description

Trypan Blue is a tetrasulfonated anionic, hydrophilic azo dye derived from toluidine. The non-permeable cell membrane dye is a vital dye that selectively stains dead or non-viable cells, that pass through damaged but not through intact cell membranes.

Application

Trypan blue applications include:

it is a routinely used for viability testing for diverse cell types including frozen sperm and aortic muscle cells exposed to antifungal agents.
employed as a collagen stain in a Van Gieson procedure
To stain amyloid, Trypan blue is considered as a blue alternative to Congo red in Puchtler-Bennhold stains.
used as a fluorescent tracer of cell populations in embryology
used as a tumor promoter modulating permeability of lysosomal membranes

Trypan Blue solution has also been used in the cytotoxity tests and to determine the vital cells for the analysis of cell division.

Biochem/physiol Actions

Trypan Blue is excluded by most living cells, but can be taken into phagocytes and certain other cells.

Physical form

0.4% in 0.81% sodium chloride; 0.06% potassium phosphate, dibasic

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pictograms

GHS08

signalword

Danger

hcodes

H350

pcodes

P201 - P202 - P280 - P308 + P313 - P405 - P501

Hazard Classifications

Carc. 1B

저장 등급

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c