InChI
1S/C6H9NO/c1-2-7-5-3-4-6(7)8/h2H,1,3-5H2
InChI key
WHNWPMSKXPGLAX-UHFFFAOYSA-N
biological source
synthetic (oragnic)
form
powder
mol wt
average mol wt 40,000
impurities
<5.0% water (Karl Fischer)
viscosity number
29-32(lit.)
solubility
water: 100 mg/mL, clear, colorless to light yellow
application(s)
agriculture
Quality Level
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General description
Polyvinylpyrrolidone (PVP) can form complexes with phenolics and alkaloids. The complex formation allows removal of phenolics and alkaloids from plant samples, thereby inhibiting any modification of proteins by them and any hindrance they may cause in spectrophotometric determinations of protein content. PVP might also enhances the stability of enzymes.
Polyvinylpyrrolidone (PVP), a multifunctional synthetic polymer, finds utility in life science and biochemical research. This water-soluble polymer is versatile, serving as a stabilizing agent, emulsifier, suspending agent, solubilizing agent, protective colloid, and complexing agent. Its protective colloid properties are particularly valuable in stabilizing proteins, enzymes, and other biological substances.
Application
Polyvinylpyrrolidone has been used in the purification of glutamine synthetases from plant samples. It has been used to block the PVDF (polyvinylidene difluoride) membrane and to avoid nonspecific binding.
Features and Benefits
- Highly versatile surfactant for your life science and biochemical research
- Excellent Solubilizing agent and Complexing agent
Other Notes
For additional information on our range of Biochemicals, please complete this form.
Polyvinylpyrrolidone is a component of Denhardt′s Solution and is included at a concentration of 1% (w/v) in the standard 50X stock solution.
저장 등급
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Glutamine Synthetases of Higher Plants : Evidence for a Specific Isoform Content Related to Their Possible Physiological Role and Their Compartmentation within the Leaf.
McNally SF et al.
Plant Physiology, 72, 22-22 (1983)
Angiotensin II inhibits insulin signaling in aortic smooth muscle cells at multiple levels. A potential role for serine phosphorylation in insulin/angiotensin II crosstalk.
Folli F et al.
The Journal of Clinical Investigation, 100, 2158-2158 (1997)
Quantification of the Antioxidant Activity in Salt-Stressed Tissues.
Maksimovic JJD and Zivanovic BD
Plant Salt Tolerance: Methods and Protocols, 237-237 (2012)
Andreas U Müller et al.
Nature communications, 10(1), 4653-4653 (2019-10-13)
In mycobacteria, transcriptional activator PafBC is responsible for upregulating the majority of genes induced by DNA damage. Understanding the mechanism of PafBC activation is impeded by a lack of structural information on this transcription factor that contains a widespread, but
Structural analysis of N- and O-glycans released from glycoproteins.
Jensen PH et al.
Nature Protocols, 7, 1299-1299 (2012)
프로토콜
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
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